Any feedback?
Literature summary for 3.1.1.26 extracted from
- Purification of a lipolytic acyl-hydrolase from Phaseolus vulgaris leaves by affinity chromatography on palmitolyated gauze and its properties (1979), Agric. Biol. Chem., 43, 563-570.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 212% activity compared to assay without additives at 0.1 mM, 198% at 1 mM, 161% at 10 mM | Phaseolus vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | no inhibition at 0.1 and 1.0 mM, 48% inhibition at 10 mM | Phaseolus vulgaris | |
| additional information | not inhibited by MgCl2 at 10 mM | Phaseolus vulgaris | |
| NaCl | 86% inhibition at 50 mM | Phaseolus vulgaris | |
| SDS | 0.1% complete inhibition | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
monogalactosyldiacylglycerol | - |
Phaseolus vulgaris | |
| 0.53 | - |
phosphatidylcholine | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
SDS-PAGE, gel filtration | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalactosyldiacylglycerol + H2O | Phaseolus vulgaris | - |
? | - |
? | |
| monogalactosyldiacylglycerol + H2O | Phaseolus vulgaris | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 88% inhibition at 10% | Phaseolus vulgaris |
| Ethanol | 98% inhibition at 10% | Phaseolus vulgaris |
| ether | 94% inhibition at 10% | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 227fold | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.655 | - |
- |
Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme preparation, 3 months stable | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digalactosyldiacylglycerol + H2O | 39% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | digalactosylglycerol + fatty acid | - |
? | |
| digalactosyldiacylglycerol + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| digalactosylmonoacylglycerol + H2O | 49% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | digalactosylglycerol + fatty acid | - |
? | |
| lysophosphatidylcholine + H2O | 89% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| lysophosphatidylethanolamine + H2O | 32% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| lysophosphatidylglycerol + H2O | 93% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| monogalactosyldiacylglycerol + H2O | best substrate | Phaseolus vulgaris | galactosylglycerol + fatty acid | - |
? | |
| monogalactosyldiacylglycerol + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
| monogalactosylmonoacylglycerol + H2O | 132% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | monogalactosylglycerol + fatty acid | - |
? | |
| phosphatidic acid + H2O | 7% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| phosphatidylcholine + H2O | 31% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| phosphatidylethanolamine + H2O | 11% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| phosphatidylglycerol + H2O | 47% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| sulfoquinovosyldiaclglycerol + H2O | 14% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
| triacylglycerol + H2O | below 0.1% of the activity with monogalactosyldiacylglycerol | Phaseolus vulgaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
monogalactosyldiaylglycerol and phosphatidylcholine as substrates | Phaseolus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
inactivated, pH 4 after 20 min, 80% of the activity retained by the same heating at pH 7.5 | Phaseolus vulgaris |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
