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Literature summary for 3.1.1.25 extracted from

  • Bayrak, A.; Bayrak, T.; Demirpence, E.; Kilinc, K.
    Differential hydrolysis of homocysteine thiolactone by purified human serum (192)Q and (192)R PON1 isoenzymes (2011), J. Chromatogr. B, 879, 49-55.
    View publication on PubMed
Search for more literature for 3.1.1.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.2
-
 homocysteine thiolactone isozyme 192R, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
23.5
-
 homocysteine thiolactone isozyme 192Q, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homocysteine thiolactone + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homocysteine thiolactone + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11
-
 homocysteine thiolactone isozyme 192Q, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens
21.2
-
 homocysteine thiolactone isozyme 192R, in 50 mM HEPES (pH 7.4), 37°C Homo sapiens