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Literature summary for 3.1.1.25 extracted from

  • Nguyen, S.D.; Hung, N.D.; Cheon-Ho, P.; Ree, K.M.; Dai-Eun, S.
    Oxidative inactivation of lactonase activity of purified human paraoxonase 1 (PON1) (2009), Biochim. Biophys. Acta, 1790, 155-160.
    View publication on PubMed
Search for more literature for 3.1.1.25

Inhibitors

Inhibitors Comment Organism Structure
ascorbate ascorbate/Cu2+ system is the most potent in inactivating the lactonase activity (by 90%). Cu2+ remarkably enhances the inactivation in the presence of ascorbate. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 95% inactivation. Ascorbate/Cu2+-mediated inactivation of lactonase activity is prevented by catalase (90%), oleic acid (73% at 0.01 mM) and dioleoylphosphatidylcholine (68% at 0.1 mM). Ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows 20% inactivation Homo sapiens
Cu2+ inhibits in a concentration-dependent manner. Cu2+ alone inhibits the lactonase activity by 30% at concentrations as low as 0.001 mM. Ascorbate/Cu2+ system is the most potent in inactivating the lactonase activity (by 90%). Cu2+ remarkably enhances the inactivation in the presence of ascorbate. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 95% inactivation. Ascorbate/Cu2+-mediated inactivation of lactonase activity is prevented by catalase (90%), oleic acid (73% at 0.01 mM) and dioleoylphosphatidylcholine (68% at 0.1 mM) Homo sapiens
Fe2+ ascorbate/Fe2+ (0.5 mM/0.002 mM) system shows 20% inactivation. Fe2+ alone shows no inhibition Homo sapiens
additional information neither ascorbate alone nor Mn2+, Zn2+, Co2+ or 3-morpholinosydnoimine cause a significant loss of lactonase activity Homo sapiens
sodium hypochlorite at 1 mM and 10 mM causes approximately 18 and 34% decrease in lactonase activity, respectively Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for the catalysis. Shows a slight protection of 10% at 5 mM on ascorbate/Cu2+-induced inactivation of lactonase activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by different types of chromatographies Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delta-valerolactone + H2O
-
 Homo sapiens 5-hydroxypentanoic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
lactonase
-
 Homo sapiens