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Literature summary for 3.1.1.25 extracted from
- Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: a robust biocatalyst for production of chiral lactones (2007), Appl. Microbiol. Biotechnol., 75, 1087-1094.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
TSK-gel column | Fusarium proliferatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium proliferatum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| process of ultrafiltration, acetone precipitation, anion-exchange, hydrophobic and hydroxyapatite chromatography | Fusarium proliferatum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0152 | - |
substrate gamma-butyrolactone | Fusarium proliferatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-hydroxy-beta,beta-dimethyl-gamma-butyrolactone + H2O | - |
Fusarium proliferatum | 2,4-dihydroxy-3,3-dimethylbutanoate | - |
? |  |
| alpha-hydroxy-gamma-butyrolactone + H2O | - |
Fusarium proliferatum | 2,4-dihydroxybutanoate | - |
? | |
| beta-butyrolactone + H2O | - |
Fusarium proliferatum | 3-hydroxybutanoate | - |
? | |
| beta-hydroxy-gamma-butyrolactone + H2O | - |
Fusarium proliferatum | 3,4-dihydroxybutanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000 Da | Fusarium proliferatum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| levo-lactonase | - |
Fusarium proliferatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
measured for gamma-butyrolactone hydrolysis | Fusarium proliferatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
measured for gamma-butyrolactone hydrolysis | Fusarium proliferatum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Fusarium proliferatum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusarium proliferatum | determined by two-dimensional electrophoresis | - |
5.7 |
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