Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.24 extracted from

  • Bains, J.; Kaufman, L.; Farnell, B.; Boulanger, M.J.
    A product analog bound form of 3-oxoadipate-enol-lactonase (PcaD) reveals a multifunctional role for the divergent cap domain (2011), J. Mol. Biol., 406, 649-658.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Paraburkholderia xenovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with levulinic acid Paraburkholderia xenovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57600
-
calculated from amino acid sequence Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans Q13KT2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography and Superdex75 gel filtration Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoadipate-enol-lactone + H2O also known as beta-ketoadipate-enol-lactone Paraburkholderia xenovorans 3-oxoadipate
-
?
4-hydroxy-3-pentenoic acid gamma-lactone + H2O
-
Paraburkholderia xenovorans 4-oxopentanoic acid i.e. levulinic acid ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Paraburkholderia xenovorans

Synonyms

Synonyms Comment Organism
3-oxoadipate-enol-lactonase
-
Paraburkholderia xenovorans
ELH
-
Paraburkholderia xenovorans
enol-lactone hydrolase
-
Paraburkholderia xenovorans
PcaD
-
Paraburkholderia xenovorans