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Literature summary for 3.1.1.23 extracted from

  • Laitinen, T.; Navia-Paldanius, D.; Rytilahti, R.; Marjamaa, J.J.; Ka?izkova, J.; Parkkari, T.; Pantsar, T.; Poso, A.; Laitinen, J.T.; Savinainen, J.R.
    Mutation of Cys242 of human monoacylglycerol lipase disrupts balanced hydrolysis of 1- and 2-monoacylglycerols and selectively impairs inhibitor potency (2014), Mol. Pharmacol., 85, 510-519.
    View publication on PubMed
Search for more literature for 3.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C201A the mutation causes a significant reduction in overall activity particularly skewing the balanced hydrolysis of monoacyl glycerol isomers to favor the 2-isomer over the 1-isomer Homo sapiens
C208A the mutation causes a significant reduction in overall activity particularly skewing the balanced hydrolysis of monoacyl glycerol isomers to favor the 2-isomer over the 1-isomer Homo sapiens
C242A the mutation causes a significant reduction in overall activity particularly skewing the balanced hydrolysis of monoacyl glycerol isomers to favor the 2-isomer over the 1-isomer Homo sapiens
Y194A the mutation causes a significant reduction in overall activity Homo sapiens
Y194A/C242A the mutation causes a significant reduction in overall activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
isopropyldodecylfluorophosphonate
-
 Homo sapiens
JJKK-048 i.e. 4-[bis-(benzo[d][1,3]dioxol-5-yl)methyl]-piperidin-1-yl}(1H-1,2,4-triazol-1-yl)methanone Homo sapiens
JZL184 i.e. 4-nitrophenyl 4-(dibenzo[d][1,3]dioxol-5-yl(hydroxy)methyl)piperidine-1-carboxylate Homo sapiens
methylarachidonoylfluorophosphonate
-
 Homo sapiens
N-arachidonoylmaleimide
-
 Homo sapiens
pristimerin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol mutant enzyme C242A, at pH 7.4 and 37°C Homo sapiens
0.013
-
 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol mutant enzyme C201A, at pH 7.4 and 37°C Homo sapiens
0.015
-
 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.016
-
 2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol mutant enzyme C208A, at pH 7.4 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-arachidonoylglycerol + H2O
-
 Homo sapiens glycerol + arachidonic acid
-
 ?
1-capryloyl-rac-glycerol + H2O
-
 Homo sapiens glycerol + caprylic acid
-
 ?
1-decanoyl-rac-glycerol + H2O best substrate Homo sapiens glycerol + decanoic acid
-
 ?
1-lauroyl-rac-glycerol + H2O
-
 Homo sapiens glycerol + lauric acid
-
 ?
1-linoleoylglycerol + H2O
-
 Homo sapiens glycerol + linoleic acid
-
 ?
1-myristoyl-rac-glycerol + H2O
-
 Homo sapiens glycerol + myristic acid
-
 ?
2-(15-deoxy-DELTA12,14-prostaglandin J2)-glycerol + H2O
-
 Homo sapiens ?
-
 ?
2-arachidonoylglycerol + H2O
-
 Homo sapiens glycerol + arachidonic acid
-
 ?
2-linoleoylglycerol + H2O
-
 Homo sapiens glycerol + linoleic acid
-
 ?
2-palmitoylglycerol + H2O
-
 Homo sapiens glycerol + palmitic acid
-
 ?
prostaglandin D2-glycerol + H2O
-
 Homo sapiens ?
-
 ?
prostaglandin E2-glycerol + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MGL
-
 Homo sapiens
monoacylglycerol lipase
-
 Homo sapiens