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Literature summary for 3.1.1.23 extracted from

  • Wang, Y.; Chanda, P.; Jones, P.G.; Kennedy, J.D.
    A fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening (2008), Assay Drug Dev. Technol., 6, 387-393.
    View publication on PubMed
Search for more literature for 3.1.1.23

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin MAGL activity increases with increasing concentration up to 1% Homo sapiens
additional information dimethyl sulfoxide concentrations up to 10% of the final assay volume have no effect on MAGL activity, the inclusion of dimethyl sulfoxide in the assay can increase the solubility of hydrophobic compounds Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into vector pColdII with a His6 tag at the N-terminus. Expression in Escherichia coli BL21 (DE3) competent cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information URB754 does not inhibit MAGL activity at concentrations up to 0.100 mM. Assay buffer alone or heat-denatured MAGL protein has no significant activity against 7-hydroxycoumarinyl-arachidonate Homo sapiens
N-arachidonyl maleimide potent irreversible inhibitor of MAGL, inhibits in a dose-dependent manner Homo sapiens
URB602 specific inhibitor of MAGL, inhibits in a dose-dependent manner Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
 7-hydroxycoumarinyl arachidonate at pH 8 and 25°C, in 10% dimethyl sulfoxide Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, MAGL-His6 protein, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MAGL-His6 protein purified on nickel-nitrilotriacetic acid column, more than 90% pure Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-hydroxycoumarinyl arachidonate
-
 Homo sapiens arachidonic acid + 7-hydroxycoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, MAGL-His6 protein, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MAGL
-
 Homo sapiens
monoacylglycerol lipase
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8 10 activity peak of 7-hydroxycoumarinyl-arachidonate hydrolysis by MAGL Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000155
-
 in 50 mM HEPES buffer, pH 8, 1 mM EDTA, and 10% dimethyl sulfoxide at 25°C for 60 min Homo sapiens N-arachidonyl maleimide
0.0031
-
 in 50 mM HEPES buffer, pH 8, 1 mM EDTA, and 10% dimethyl sulfoxide at 25°C for 60 min Homo sapiens URB602