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Literature summary for 3.1.1.23 extracted from

  • Dinh, T.P.; Kathuria, S.; Piomelli, D.
    RNA interference suggests a primary role for monoacylglycerol lipase in the degradation of the endocannabinoid 2-arachidonoylglycerol (2004), Mol. Pharmacol., 66, 1260-1264.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of Rattus norvegicus brain enzyme in HeLa cells Homo sapiens
functional overexpression of the enzyme in HeLa cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of a HeLa cell line, HeLa-MGLi, with stable repression of enzyme expression by RNAi silencing, the cells show 20% of wild-type cell line enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
methyl arachidonyl fluorophosphonate i.e. MAFP Homo sapiens
additional information immunodepletion of the enzyme leads to 50% reduced activity Homo sapiens
additional information immunodepletion of the recombinant enzyme leads to 50% reduced activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE Rattus norvegicus
37000
-
x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-arachidonoylglycerol + H2O Homo sapiens the enzyme is responsible for degradation of 2-arachidonoylglycerol in HeLa cells arachidonic acid + glycerol
-
?
2-arachidonoylglycerol + H2O Rattus norvegicus the enzyme is responsible for degradation of 2-arachidonoylglycerol in the brain arachidonic acid + glycerol
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain 2 isozymes from alternative slicing Rattus norvegicus
-
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-arachidonoylglycerol + H2O
-
Homo sapiens arachidonic acid + glycerol
-
?
2-arachidonoylglycerol + H2O
-
Rattus norvegicus arachidonic acid + glycerol
-
?
2-arachidonoylglycerol + H2O the enzyme is responsible for degradation of 2-arachidonoylglycerol in HeLa cells Homo sapiens arachidonic acid + glycerol
-
?
2-arachidonoylglycerol + H2O the enzyme is responsible for degradation of 2-arachidonoylglycerol in the brain Rattus norvegicus arachidonic acid + glycerol
-
?

Subunits

Subunits Comment Organism
? x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
MGL
-
Homo sapiens
MGL
-
Rattus norvegicus
monoacylglycerol lipase
-
Homo sapiens
monoacylglycerol lipase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens
8
-
assay at Rattus norvegicus