Cloned (Comment) | Organism |
---|---|
functional overexpression of Rattus norvegicus brain enzyme in HeLa cells | Homo sapiens |
functional overexpression of the enzyme in HeLa cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a HeLa cell line, HeLa-MGLi, with stable repression of enzyme expression by RNAi silencing, the cells show 20% of wild-type cell line enzyme activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methyl arachidonyl fluorophosphonate | i.e. MAFP | Homo sapiens | |
additional information | immunodepletion of the enzyme leads to 50% reduced activity | Homo sapiens | |
additional information | immunodepletion of the recombinant enzyme leads to 50% reduced activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE | Rattus norvegicus |
37000 | - |
x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-arachidonoylglycerol + H2O | Homo sapiens | the enzyme is responsible for degradation of 2-arachidonoylglycerol in HeLa cells | arachidonic acid + glycerol | - |
? | |
2-arachidonoylglycerol + H2O | Rattus norvegicus | the enzyme is responsible for degradation of 2-arachidonoylglycerol in the brain | arachidonic acid + glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | 2 isozymes from alternative slicing | Rattus norvegicus | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-arachidonoylglycerol + H2O | - |
Homo sapiens | arachidonic acid + glycerol | - |
? | |
2-arachidonoylglycerol + H2O | - |
Rattus norvegicus | arachidonic acid + glycerol | - |
? | |
2-arachidonoylglycerol + H2O | the enzyme is responsible for degradation of 2-arachidonoylglycerol in HeLa cells | Homo sapiens | arachidonic acid + glycerol | - |
? | |
2-arachidonoylglycerol + H2O | the enzyme is responsible for degradation of 2-arachidonoylglycerol in the brain | Rattus norvegicus | arachidonic acid + glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, brain splicing variant isozyme 1, SDS-PAGE, x * 37000, brain splicing variant isozyme 1, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
MGL | - |
Homo sapiens |
MGL | - |
Rattus norvegicus |
monoacylglycerol lipase | - |
Homo sapiens |
monoacylglycerol lipase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Rattus norvegicus |