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Literature summary for 3.1.1.22 extracted from

  • Zhang, K.; Shiraki, M.; Saito, T.
    Purification of an extracellular D-(-)-3-hydroxybutyrate oligomer hydrolase from Pseudomonas sp. strain A1 and cloning and sequencing of its gene (1997), J. Bacteriol., 179, 72-77.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Pseudomonas sp.
0.18
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 1 * 70000, SDS-PAGE Pseudomonas sp.
72880
-
 calculation from sequence of cDNA Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate Pseudomonas sp. degradation of poly-(3-hydroxybutyrate) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 strain a
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate degradation of poly-(3-hydroxybutyrate) Pseudomonas sp. ?
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
D-(-)3-(3-O-methyloxybutyryl)hydroxybutyrate + H2O
-
 Pseudomonas sp. ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Pseudomonas sp. p-nitrophenol + acetate
-
 ?
p-nitrophenyl butyrate + H2O
-
 Pseudomonas sp. p-nitrophenol + butanoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Pseudomonas sp.