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Literature summary for 3.1.1.22 extracted from

  • Tanaka, Y.; Saito, T.; Fukui, T.; Tanio, T.; Tomita, K.
    Purification and properties of D(-)-3-hydroxybutyrate-dimer hydrolase from Zoogloea ramigera I-16-M (1981), Eur. J. Biochem., 118, 177-182.
    View publication on PubMed
Search for more literature for 3.1.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 (R)-3-((R)-3-hydroxybutanoyloxy)4butanoate
-
 Zoogloea ramigera
0.33
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Zoogloea ramigera
0.36
-
 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
-
 Zoogloea ramigera
0.59
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Zoogloea ramigera

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Zoogloea ramigera
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000 30000 gel filtration, SDS-PAGE Zoogloea ramigera
30000
-
 1 * 30000, SDS-PAGE Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate Zoogloea ramigera degradation of poly-(3-hydroxybutyrate) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zoogloea ramigera

Storage Stability

Storage Stability Organism
-20°C, pH 8.0, several months Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
-
 Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate degradation of poly-(3-hydroxybutyrate) Zoogloea ramigera ?
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O attack from th free hydroxyl terminus, releasing monomer units one at a time Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
-
 Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
-
 Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid Zoogloea ramigera (R)-3-hydroxybutanoate
-
 ?
D-(-)-3-butyryloxybutyrate + H2O
-
 Zoogloea ramigera ?
-
 ?
D-(-)3-(3-O-methyloxybutyryl)hydroxybutyrate + H2O
-
 Zoogloea ramigera ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Zoogloea ramigera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Zoogloea ramigera