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Literature summary for 3.1.1.22 extracted from

  • Lu, J.; Takahashi, A.; Ueda, S.
    3-Hydroxybutyrate oligomer hydrolase and 3-hydroxybutyrate dehydrogenase participate in intracellular polyhydroxybutyrate and polyhydroxyvalerate degradation in Paracoccus denitrificans (2014), Appl. Environ. Microbiol., 80, 986-993.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Paracoccus denitrificans

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate completely inhibited by 1 mM diisopropylfluorophosphate Paracoccus denitrificans
iodoacetamide 74.8% residual activity at 1 mM Paracoccus denitrificans
N-ethylmaleimide 74.8% residual activity at 1 mM Paracoccus denitrificans
phenylmethylsulfonyl fluoride 27.4% residual activity at 1 mM Paracoccus denitrificans
SDS 61.1% residual activity at 0.05% (w/v) Paracoccus denitrificans
Triton X-100 67.3% residual activity at 0.05% (v/v) Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 4 * 31000, SDS-PAGE Paracoccus denitrificans
120000
-
 gel filtration Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans V5YT97
-
-
Paracoccus denitrificans PD01 V5YT97
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and TSK gel G3000SW gel filtration Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
 with 3-(3-hydroxypentanoyloxy)pentanoate as substrate, at pH 8.0 and 30°C Paracoccus denitrificans
0.67
-
 with 3-(3-hydroxybutanoyloxy)butanoate as substrate, at pH 8.0 and 30°C Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(3-hydroxybutanoyloxy)butanoate + H2O
-
 Paracoccus denitrificans 3-hydroxybutanoate
-
 ?
3-(3-hydroxybutanoyloxy)butanoate + H2O
-
 Paracoccus denitrificans PD01 3-hydroxybutanoate
-
 ?
3-(3-hydroxypentanoyloxy)pentanoate + H2O
-
 Paracoccus denitrificans 3-hydroxypentanoate
-
 ?
3-(3-hydroxypentanoyloxy)pentanoate + H2O
-
 Paracoccus denitrificans PD01 3-hydroxypentanoate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Paracoccus denitrificans 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Paracoccus denitrificans PD01 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Paracoccus denitrificans 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Paracoccus denitrificans PD01 4-nitrophenol + pentanoate
-
 ?
additional information pHB and PHV granules are not depolymerized by the enzyme Paracoccus denitrificans ?
-
 ?
additional information pHB and PHV granules are not depolymerized by the enzyme Paracoccus denitrificans PD01 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 31000, SDS-PAGE Paracoccus denitrificans

Synonyms

Synonyms Comment Organism
3-hydroxybutyrate oligomer hydrolase
-
 Paracoccus denitrificans
PhaZc
-
 Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paracoccus denitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 more than 50% activity between 20-60°C Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Paracoccus denitrificans

pH Range

pH Minimum pH Maximum Comment Organism
5 9 more than 50% activity between pH 5.0 and 9.0 Paracoccus denitrificans