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Literature summary for 3.1.1.20 extracted from
- Studies on fungal tannase. Part I. Formation, purification and catalytic properties of tannase of Aspergillus flavus (1968), Agric. Biol. Chem., 32, 1070-1078.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Tannic acid | - |
Aspergillus flavus |  |
| 0.14 | - |
1-O-Galloyl-beta-D-glucose | - |
Aspergillus flavus | |
| 0.86 | - |
methyl gallate | - |
Aspergillus flavus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Aspergillus niger | - |
? | - |
? | |
| tannic acid + H2O | Penicillium chrysogenum | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus flavus | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus oryzae | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus sojae | - |
? | - |
? | |
| tannic acid + H2O | Penicillium oxalicum | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus awamori | - |
? | - |
? | |
| tannic acid + H2O | Penicillium expansum | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus ustus | - |
? | - |
? | |
| tannic acid + H2O | Aspergillus usamii | - |
? | - |
? | |
| tannic acid + H2O | Penicillium javanicum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus flavus | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus sojae | - |
- |
- |
| Aspergillus usamii | - |
- |
- |
| Aspergillus ustus | - |
- |
- |
| Penicillium chrysogenum | - |
- |
- |
| Penicillium expansum | - |
- |
- |
| Penicillium javanicum | - |
- |
- |
| Penicillium oxalicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 30fold | Aspergillus flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 135 | - |
5 step purification | Aspergillus flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-O-galloyl-beta-D-glucose + H2O | - |
Aspergillus flavus | gallic acid + beta-D-glucose | - |
? | |
| methyl gallate + H2O | - |
Aspergillus flavus | gallic acid + methanol | - |
? | |
| methyl gallate + H2O | - |
Aspergillus oryzae | gallic acid + methanol | - |
? | |
| tannic acid + H2O | - |
Aspergillus niger | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Penicillium chrysogenum | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus flavus | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus sojae | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Penicillium oxalicum | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus awamori | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Penicillium expansum | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus ustus | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus usamii | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Penicillium javanicum | gallic acid + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus flavus | 10 gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae | 10 gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
| tannic acid + H2O | - |
Penicillium chrysogenum | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus flavus | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus sojae | ? | - |
? | |
| tannic acid + H2O | - |
Penicillium oxalicum | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus awamori | ? | - |
? | |
| tannic acid + H2O | - |
Penicillium expansum | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus ustus | ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus usamii | ? | - |
? | |
| tannic acid + H2O | - |
Penicillium javanicum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Aspergillus flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Aspergillus flavus |
| 70 | - |
- |
Aspergillus oryzae |
| 70 | - |
the activity is rapidly lost | Aspergillus flavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | methyl gallate as substrate | Aspergillus flavus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | methyl gallate as substrate, stable in 0.01 M acetate buffer, at 5°C, outside this range the activity increases rapidly | Aspergillus flavus |
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