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Literature summary for 3.1.1.20 extracted from

  • Ristinmaa, A.S.; Coleman, T.; Cesar, L.; Langborg Weinmann, A.; Mazurkewich, S.; Braenden, G.; Hasani, M.; Larsbrink, J.
    Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum (2022), J. Biol. Chem., 298, 101758.
    View publication on PubMed
Search for more literature for 3.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 2 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
2 3 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
2 2.2 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
2 3.4 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.5
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
7.2
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
9
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
10.8
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
24.3
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucogallin + H2O
-
 Clostridium butyricum gallate + D-glucose
-
 ?
hexyl gallate + H2O
-
 Clostridium butyricum gallate + hexanol
-
 ?
methyl gallate + H2O
-
 Clostridium butyricum gallate + methanol
-
 ?
oak bark extract + H2O
-
 Clostridium butyricum ?
-
 ?
propyl gallate + H2O
-
 Clostridium butyricum gallate + propanol
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, isoform Tan1, x * 55000, isoform Tan2, SDS-PAGE Clostridium butyricum

Synonyms

Synonyms Comment Organism
K670DRAFT_03878 locus tag Clostridium butyricum
K670DRAFT_04016 locus tag Clostridium butyricum
K670DRAFT_04079 locus tag Clostridium butyricum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.9
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
26.9
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
32
-
 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
45.9
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
136
-
 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
187
-
 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
209
-
 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
213
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
459
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 isoforms Tan1, Tan2 Clostridium butyricum
7
-
 isoform Tan3 Clostridium butyricum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
2.2
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
2.4
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
2.6
-
 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
6.2
-
 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
8
-
 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
10.2
-
 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
52.9
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
72.8
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum