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Literature summary for 3.1.1.20 extracted from

  • Mahmoud, A.E.; Fathy, S.A.; Rashad, M.M.; Ezz, M.K.; Mohammed, A.T.
    Purification and characterization of a novel tannase produced by Kluyveromyces marxianus using olive pomace as solid support, and its promising role in gallic acid production (2018), Int. J. Biol. Macromol., 107, 2342-2350 .
    View publication on PubMed
Search for more literature for 3.1.1.20

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 27% inhibition at 1 mM Kluyveromyces marxianus
Co2+ 32% inhibition at 1 mM Kluyveromyces marxianus
Hg2+ 57% inhibition at 1 mM Kluyveromyces marxianus
K+ 58% inhibition at 1 mM Kluyveromyces marxianus
Mg2+ 10% inhibition at 1 mM Kluyveromyces marxianus
Mn2+ 60% inhibition at 1 mM Kluyveromyces marxianus
Na+ 30% inhibition at 1 mM Kluyveromyces marxianus
Zn2+ 22% inhibition at 1 mM Kluyveromyces marxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
 Tannic acid pH pH 5.5, 30°C Kluyveromyces marxianus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 12% activation at 1 mM Kluyveromyces marxianus
Cd2+ 42.2% activation at 1 mM Kluyveromyces marxianus
Cu2+ 35.8% activation at 1 mM Kluyveromyces marxianus
Fe3+ 28.5% activation at 1 mM Kluyveromyces marxianus
Li+ 14% activation at 1 mM Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66620
-
 gel filtration Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Kluyveromyces marxianus
-
 gallic acid + ?
-
 ?
tannic acid + H2O Kluyveromyces marxianus NRRL Y-8281
-
 gallic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
-
-
Kluyveromyces marxianus NRRL Y-8281
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 24.21fold by ammonium sulfate fractionation and gel filtration Kluyveromyces marxianus

Source Tissue

Source Tissue Comment Organism Textmining
additional information culture growth on olive pomace as solid support in solid state fermentation Kluyveromyces marxianus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1026.12
-
 purified native enzyme, pH 5.5, 30°C Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epigallocatechin gallate + H2O
-
 Kluyveromyces marxianus gallic acid + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 gallic acid + epigallocatechin
-
 ?
methyl gallate + H2O
-
 Kluyveromyces marxianus methanol + gallic acid
-
 ?
methyl gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 methanol + gallic acid
-
 ?
additional information the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) Kluyveromyces marxianus ?
-
 ?
additional information the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) Kluyveromyces marxianus NRRL Y-8281 ?
-
 ?
propyl gallate + H2O
-
 Kluyveromyces marxianus propanol + gallic acid
-
 ?
propyl gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 propanol + gallic acid
-
 ?
tannic acid + H2O
-
 Kluyveromyces marxianus gallic acid + ?
-
 ?
tannic acid + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 gallic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Kluyveromyces marxianus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity profile overview Kluyveromyces marxianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 purified native enzyme, thermostable within the temperature range 30-70°C, 30 min Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 optimum 1 Kluyveromyces marxianus
8.5
-
 optimum 2 Kluyveromyces marxianus

pH Range

pH Minimum pH Maximum Comment Organism
3 9 activity profile overview Kluyveromyces marxianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5.5 purified native enzyme, stable in the pH range of pH 4.0-5.5 for 30 min Kluyveromyces marxianus