Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.20 extracted from

  • Wang, D.; Liu, Y.; Lv, D.; Hu, X.; Zhong, Q.; Zhao, Y.; Wu, M.
    Substrates specificity of tannase from Streptomyces sviceus and Lactobacillus plantarum (2018), AMB Express, 8, 147 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene tanLpl, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Lactiplantibacillus plantarum
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces sviceus

Protein Variants

Protein Variants Comment Organism
D453A site-directed mutagenesis Streptomyces sviceus
D455A site-directed mutagenesis Streptomyces sviceus
E385A site-directed mutagenesis Streptomyces sviceus
G384P site-directed mutagenesis, the mutant shows 138.1% esterase activity compared to wild-type, and a lower depsidase activity (61.1%) Streptomyces sviceus
H485A site-directed mutagenesis Streptomyces sviceus
K371A site-directed mutagenesis Streptomyces sviceus
additional information an enzyme variant lacking the flap-like domain shows reduced esterase (68%) and highly increased depsidase (244%) activity compared to the wild-type enzyme Lactiplantibacillus plantarum
additional information an enzyme variant lacking the flap-like domain shows slightly reduced esterase and reduced depsidase activity compared to the wild-type enzyme Streptomyces sviceus
P356G site-directed mutagenesis, the mutant P356G only has 48.2% esterase activity compared to wild-type, while it shows a higher depsidase activity (131.7%) than the wild-type enzyme Lactiplantibacillus plantarum
S210A site-directed mutagenesis Streptomyces sviceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Lineweaver-Burk kinetics Lactiplantibacillus plantarum
additional information
-
 additional information Lineweaver-Burk kinetics Streptomyces sviceus
0.21
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus
0.41
-
 Tannic acid pH 7.0, 37°C, recombinant mutant P356G Streptomyces sviceus
0.53
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum
0.58
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus
0.67
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
0.94
-
 Tannic acid pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
1.1
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum
1.23
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
1.3
-
 methyl gallate pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
2.4
-
 methyl gallate pH 7.0, 37°C, recombinant mutant G384P Streptomyces sviceus
3.3
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus
3.5
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum B3Y018
-
-
Streptomyces sviceus A0A077YGC3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Lactiplantibacillus plantarum
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptomyces sviceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
 Lactiplantibacillus plantarum methanol + gallic acid
-
 ?
methyl gallate + H2O
-
 Streptomyces sviceus methanol + gallic acid
-
 ?
additional information wild-type enzyme LP-tan has a higher esterase activity than depsidase activity Lactiplantibacillus plantarum ?
-
 ?
additional information wild-type enzyme SS-tan has a higher depsidase activity than esterase activity Streptomyces sviceus ?
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallic acid + ?
-
 ?
tannic acid + H2O
-
 Streptomyces sviceus gallic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, C-terminally His-tagged enzyme, SDS-PAGE Lactiplantibacillus plantarum
? x * 51000, C-terminally His-tagged enzyme, SDS-PAGE Streptomyces sviceus
More the enzyme LP-tan displays alpha/beta structure, featured by a large cap domain inserted into the classical serine hydrolase fold, which is familiar with the feruloyl esterase and lipases. Structure comparisons, overview Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
depsidase
-
 Lactiplantibacillus plantarum
depsidase
-
 Streptomyces sviceus
LP-tan
-
 Lactiplantibacillus plantarum
TanLpl
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Lactiplantibacillus plantarum
50
-
-
 Streptomyces sviceus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60.7
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus
62.6
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
64.7
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus
66.9
-
 Tannic acid pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
69.2
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum
69.9
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus
70.3
-
 methyl gallate pH 7.0, 37°C, recombinant mutant P356G Streptomyces sviceus
79.8
-
 methyl gallate pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
85.1
-
 Tannic acid pH 7.0, 37°C, recombinant mutant G384P Streptomyces sviceus
99.4
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
104.6
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus
115.5
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lactiplantibacillus plantarum
8
-
-
 Streptomyces sviceus

General Information

General Information Comment Organism
additional information the enzyme LP-tan forms a flap domain (amino acids 225-247) and a sandwich structure (Ile206-substrate-Pro356), functional role of sandwich structure and the flap (flap-like) domain in the substrate specificity of tannase, overview. The sandwich and the flap domain can help in catalytic hydrolysis of ester bonds Lactiplantibacillus plantarum
additional information the enzyme SS-tan forms a sandwich structure (Ile253-substrate-Gly384), but no flap domain, it forms a flap-like domain (amino acids: 93-143) instead, functional role of sandwich structure and the flap (flap-like) domain in the substrate specificity of tannase, overview. The sandwich and the flap domain can help in catalytic hydrolysis of ester bonds, while the flap-like domain in SS-tan mainly works on depside bonds Streptomyces sviceus
physiological function tannases can catalyze the hydrolysis of galloyl ester and depside bonds of hydrolysable tannins to release gallic acid and glucose, but tannases from different species have different substrate specificities. The enzymes can also show depsidase activity Lactiplantibacillus plantarum
physiological function tannases can catalyze the hydrolysis of galloyl ester and depside bonds of hydrolysable tannins to release gallic acid and glucose, but tannases from different species have different substrate specificities. The enzymes can also show depsidase activity Streptomyces sviceus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17.3
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus
21.2
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus
29.3
-
 methyl gallate pH 7.0, 37°C, recombinant mutant P356G Streptomyces sviceus
50.9
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
61.4
-
 methyl gallate pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
71.2
-
 Tannic acid pH 7.0, 37°C, recombinant mutant P356G Lactiplantibacillus plantarum
105
-
 methyl gallate pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum
130.6
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant Lactiplantibacillus plantarum
148.4
-
 methyl gallate pH 7.0, 37°C, recombinant wild-type enzyme Lactiplantibacillus plantarum
180.4
-
 Tannic acid pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant Streptomyces sviceus
207.6
-
 Tannic acid pH 7.0, 37°C, recombinant mutant G384P Streptomyces sviceus
308.1
-
 Tannic acid pH 7.0, 37°C, recombinant wild-type enzyme Streptomyces sviceus