Literature summary for 3.1.1.20 extracted from

Yao, J.; Chen, Q.; Zhong, G.; Cao, W.; Yu, A.; Liu, Y.
Immobilization and characterization of tannase from a metagenomic library and its use for removal of tannins from green tea infusion (2014), J. Microbiol. Biotechnol., 24, 80-86.

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Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	1 mM EDTA leads to 106% activity of the immobilized enzyme	uncultured bacterium
Urea	1 mM urea leads to 106% and 113% activity for the free and immobilized enzyme, respectively	uncultured bacterium

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	uncultured bacterium

General Stability

General Stability	Organism
the immobilized enzyme retains its initial activity over 10 cycles and retains 80% of its initial activity after 26 cycles. After that, the activity of the immobilized enzyme is quickly lost, retaining about 40% of its initial activity after 46 cycles	uncultured bacterium

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	complete inhibition of the free enzyme at 1 mM, the immobilized enzyme shows 93.7% residual activity at 1 mM	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	1 mM Ca2+ leads to 126% and 113% activity for the free and immobilized enzyme, respectively	uncultured bacterium
Co2+	1 mM Co2+ leads to 105% activity of the immobilized enzyme	uncultured bacterium
Mg2+	1 mM Mg2+ leads to 120% and 110% activity for the free and immobilized enzyme, respectively	uncultured bacterium
Zn2+	1 mM Zn2+ leads to 108% activity of the immobilized enzyme	uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tannic acid + H2O	uncultured bacterium	-	gallate + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA His-Bind resin column chromatography	uncultured bacterium

Storage Stability

Storage Stability	Organism
4°C, free enzyme in 100 mM phosphate buffer (pH 6.5), 16 days, complete loss of activity	uncultured bacterium
4°C, immobilized enzyme in 100 mM phosphate buffer (pH 6.5), 30 days, less than 10% loss of activity	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
propyl gallate + H2O	-	uncultured bacterium	gallate + propanol	-	?
tannic acid + H2O	-	uncultured bacterium	gallate + D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
Tan410	-	uncultured bacterium
tannin acyl hydrolase	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	free enzyme	uncultured bacterium
45	-	immobilized enzyme	uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	the enzyme is the most active after incubating at 25°C for 12 h	uncultured bacterium
50	-	the immobilized enzyme retains about 75% of its initial activity after incubation at 50°C for 4 h, whereas the free enzyme retains 50% of its initial activity when exposed to the same condition	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.4	-	free enzyme	uncultured bacterium
7	-	immobilized enzyme	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	more than 50% activity between pH 5.0 and 8.0	uncultured bacterium

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Release 2023.1 (January 2023)