Any feedback?
Literature summary for 3.1.1.20 extracted from
- Purification and characterization of tannin acyl hydrolase produced by mixed solid state fermentation of wheat bran and marigold flower by Penicillium notatum NCIM 923 (2013), BioMed Res. Int., 2013, 596380.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
Tannic acid | purified enzyme, at pH 5.0 and 37°C | Penicillium chrysogenum |  |
| 6.6 | - |
Tannic acid | crude enzyme, at pH 5.0 and 37°C | Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 97000 + 1 * 43000, SDS-PAGE | Penicillium chrysogenum |
| 97000 | - |
1 * 97000 + 1 * 43000, SDS-PAGE | Penicillium chrysogenum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digallate + H2O | Penicillium chrysogenum | - |
gallate | - |
? | |
| digallate + H2O | Penicillium chrysogenum NCIM 923 | - |
gallate | - |
? | |
| tannic acid + H2O | Penicillium chrysogenum | - |
gallate + D-glucose | - |
? | |
| tannic acid + H2O | Penicillium chrysogenum NCIM 923 | - |
gallate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
| Penicillium chrysogenum NCIM 923 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE cellulose column chromatography | Penicillium chrysogenum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| digallate + H2O | - |
Penicillium chrysogenum | gallate | - |
? | |
| digallate + H2O | - |
Penicillium chrysogenum NCIM 923 | gallate | - |
? | |
| epigallocatechin gallate + H2O | - |
Penicillium chrysogenum | gallate + epigallocatechin | - |
? | |
| epigallocatechin gallate + H2O | - |
Penicillium chrysogenum NCIM 923 | gallate + epigallocatechin | - |
? | |
| gallotannin + H2O | - |
Penicillium chrysogenum | gallate + D-glucose | - |
? | |
| gallotannin + H2O | - |
Penicillium chrysogenum NCIM 923 | gallate + D-glucose | - |
? | |
| methyl gallate + H2O | - |
Penicillium chrysogenum | gallate + methanol | - |
? | |
| methyl gallate + H2O | - |
Penicillium chrysogenum NCIM 923 | gallate + methanol | - |
? | |
| tannic acid + H2O | - |
Penicillium chrysogenum | gallate + D-glucose | - |
? | |
| tannic acid + H2O | - |
Penicillium chrysogenum NCIM 923 | gallate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 97000 + 1 * 43000, SDS-PAGE | Penicillium chrysogenum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | - |
Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | after 12 h incubation, above 95% activity is retained at 40°C, while the enzyme retains more than 60% of its activity at 60°C | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | after 12 h incubation, the enzyme retains 40% activity at pH 3.0 and 8.0, respectively | Penicillium chrysogenum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
