Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.20 extracted from

  • Goncalves, H.; Riul, A.; Terenzi, H.; Jorge, J.; Guimaraes, L.
    Extracellular tannase from Emericella nidulans showing hypertolerance to temperature and organic solvents (2011), J. Mol. Catal. B, 71, 29-35.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
SDS 138.96% activity at 0.01% (v/v) Aspergillus nidulans
Triton X-100 121.63% activity at 0.01% (v/v) Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 98.77% residual activity at 1 mM Aspergillus nidulans
Al3+ 75.14% residual activity at 1 mM Aspergillus nidulans
beta-mercaptoethanol 37.07% residual activity at 20 mM Aspergillus nidulans
EDTA 25.89% residual activity at 20 mM Aspergillus nidulans
Fe3+ 85.74% residual activity at 1 mM Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.78
-
methyl gallate pH 5.0, 45°C Aspergillus nidulans
7.69
-
propyl gallate pH 5.0, 45°C Aspergillus nidulans
14.01
-
Tannic acid pH 5.0, 45°C Aspergillus nidulans
16.94
-
pyrogallol pH 5.0, 45°C Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus nidulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 117.94% activity at 1 mM Aspergillus nidulans
Ca2+ 109.51% activity at 1 mM Aspergillus nidulans
Co2+ 133.59% activity at 1 mM Aspergillus nidulans
Cu2+ 108.44% activity at 1 mM Aspergillus nidulans
Hg2+ 137.73% activity at 1 mM Aspergillus nidulans
K+ 115.18% activity at 1 mM Aspergillus nidulans
Mg2+ 118.25% activity at 1 mM Aspergillus nidulans
Mn2+ 115.8% activity at 1 mM Aspergillus nidulans
Na+ 124.69% activity at 1 mM Aspergillus nidulans
NH4Cl 110.74% activity at 1 mM Aspergillus nidulans
Zn2+ 142% activity at 1 mM Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45800
-
2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans
52000
-
2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans
302000
-
gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Aspergillus nidulans
-
gallic acid + D-glucose
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 130.37% activity at 1% (v/v) Aspergillus nidulans
acetonitrile 149.85% activity at 1% (v/v) Aspergillus nidulans
Ethanol 148.47% activity at 1% (v/v) Aspergillus nidulans
Glycerol 138.5% activity at 1% (v/v) Aspergillus nidulans
isopropanol 150% activity at 1% (v/v) Aspergillus nidulans
Methanol 139.57% activity at 1% (v/v) Aspergillus nidulans
n-Butanol 140.95% activity at 1% (v/v) Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
synonym Aspergillus nidulans
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 50% Aspergillus nidulans

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.031
-
crude extract, pH 5.0, 45°C Aspergillus nidulans
1.91
-
after 61.61fold purification, pH 5.0, 45°C Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
Aspergillus nidulans gallate + methanol
-
?
propyl gallate + H2O
-
Aspergillus nidulans gallate + propanol
-
?
pyrogallol + H2O
-
Aspergillus nidulans ?
-
?
tannic acid + H2O
-
Aspergillus nidulans gallic acid + D-glucose
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22 90 the enzyme is very stable in the temperature range of 22-50°C for up to 70 h and has a half-life of about 72 h at 90°C Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus nidulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 the enzyme retained around 80% of control activity when maintained for 60 h at pH 4.0 or 5.0. Increasing pH values (6.0-10.0) result in decreased stability of the enzyme, with a half-life of 20 h at pH 7.0 Aspergillus nidulans

Expression

Organism Comment Expression
Aspergillus nidulans enzyme production is drastically inhibited at higher concentrations of D-glucose (above 0.5% w/v) down
Aspergillus nidulans intracellular enzyme levels increase 2fold in the presence of 0.75% (w/v) gallic acid, whereas the addition of low concentrations of D-glucose (0.1% w/v) increase both extra- and intracellular activities up