Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 7% inhibition of tannase produced under submerged fermentation at 1 mM | Aspergillus niger | |
Cu2+ | 41% inhibition of tannase produced under solid-state fermentation at 1 mM | Aspergillus niger | |
Fe2+ | 63% inhibition of tannase produced under submerged fermentation at 1 mM | Aspergillus niger | |
K+ | - |
Aspergillus niger | |
Mg2+ | - |
Aspergillus niger | |
Mn2+ | - |
Aspergillus niger | |
Zn2+ | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
Tannic acid | enzyme produced under submerged fermentation, at pH 7.0, 30°C | Aspergillus niger | |
0.78 | - |
methyl gallate | enzyme produced under solid-state fermentation, at pH 7.0, 30°C | Aspergillus niger | |
1.4 | - |
Tannic acid | enzyme produced under solid-state fermentation, at pH 7.0, 30°C | Aspergillus niger | |
7.41 | - |
methyl gallate | enzyme produced under submerged fermentation, at pH 7.0, 30°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE | Aspergillus niger |
105000 | - |
x * 105000, enzyme produced under submerged fermentation, SDS-PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Aspergillus niger | - |
gallic acid + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus niger |
Purification (Comment) | Organism |
---|---|
isoelectric focusing, High Q column chromatography, High Trap Q column chromatography, High S column chromatography, and Superdex G-200 gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
264.5 | - |
enzyme produced under solid-state fermentation, after 153fold purification, at pH 7.0, 30°C | Aspergillus niger |
2067 | - |
enzyme produced under submerged fermentation, after 477fold purification, at pH 7.0, 30°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Aspergillus niger | gallate + methanol | - |
? | |
tannic acid + H2O | - |
Aspergillus niger | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE | Aspergillus niger |
? | x * 105000, enzyme produced under submerged fermentation, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
tannin acyl hydrolase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | enzyme produced under submerged fermentation | Aspergillus niger |
60 | - |
enzyme produced under solid-state fermentation | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | solid-state fermentation tannase and submerged fermentation tannase are stable at 60°C for 1 h incubation retaining around 90% of activity. Both purified tannases lose the activity at 80°C, nevertheless, the submerged fermentation tannase only loses 20% of activity at 70°C for 1 h incubation and solid-state fermentation tannase retaining 30% of activity at the same condition | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
a minor secondary peak at pH 4.0 is found in both enzymes produced under solid-state and submerged fermentations with 70% of tannase activation | Aspergillus niger |
6 | - |
pH optimum for both enzymes produced under solid-state and submerged fermentations | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | the stability pH is 6.0 at 4°C for 24 h for both purified tannases maintaining 100% of enzymatic activity. Submerged fermentation tannase supports 90-100% enzymatic activity at pH 5.0-7.0. The solid-state fermentation tannase at pH values of 5.0 and 6.0, supports 85-100% activity | Aspergillus niger |