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Literature summary for 3.1.1.20 extracted from

  • Renovato, J.; Gutierrez-Sanchez, G.; Rodriguez-Duran, L.V.; Bergman, C.; Rodriguez, R.; Aguilar, C.N.
    Differential properties of Aspergillus niger tannase produced under solid-state and submerged fermentations (2011), Appl. Biochem. Biotechnol., 165, 382-395.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 7% inhibition of tannase produced under submerged fermentation at 1 mM Aspergillus niger
Cu2+ 41% inhibition of tannase produced under solid-state fermentation at 1 mM Aspergillus niger
Fe2+ 63% inhibition of tannase produced under submerged fermentation at 1 mM Aspergillus niger
K+
-
Aspergillus niger
Mg2+
-
Aspergillus niger
Mn2+
-
Aspergillus niger
Zn2+
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
Tannic acid enzyme produced under submerged fermentation, at pH 7.0, 30°C Aspergillus niger
0.78
-
methyl gallate enzyme produced under solid-state fermentation, at pH 7.0, 30°C Aspergillus niger
1.4
-
Tannic acid enzyme produced under solid-state fermentation, at pH 7.0, 30°C Aspergillus niger
7.41
-
methyl gallate enzyme produced under submerged fermentation, at pH 7.0, 30°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE Aspergillus niger
105000
-
x * 105000, enzyme produced under submerged fermentation, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Aspergillus niger
-
gallic acid + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
isoelectric focusing, High Q column chromatography, High Trap Q column chromatography, High S column chromatography, and Superdex G-200 gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
264.5
-
enzyme produced under solid-state fermentation, after 153fold purification, at pH 7.0, 30°C Aspergillus niger
2067
-
enzyme produced under submerged fermentation, after 477fold purification, at pH 7.0, 30°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
Aspergillus niger gallate + methanol
-
?
tannic acid + H2O
-
Aspergillus niger gallic acid + D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 102000, enzyme produced under solid-state fermentation, SDS-PAGE Aspergillus niger
? x * 105000, enzyme produced under submerged fermentation, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 enzyme produced under submerged fermentation Aspergillus niger
60
-
enzyme produced under solid-state fermentation Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 solid-state fermentation tannase and submerged fermentation tannase are stable at 60°C for 1 h incubation retaining around 90% of activity. Both purified tannases lose the activity at 80°C, nevertheless, the submerged fermentation tannase only loses 20% of activity at 70°C for 1 h incubation and solid-state fermentation tannase retaining 30% of activity at the same condition Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
a minor secondary peak at pH 4.0 is found in both enzymes produced under solid-state and submerged fermentations with 70% of tannase activation Aspergillus niger
6
-
pH optimum for both enzymes produced under solid-state and submerged fermentations Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 the stability pH is 6.0 at 4°C for 24 h for both purified tannases maintaining 100% of enzymatic activity. Submerged fermentation tannase supports 90-100% enzymatic activity at pH 5.0-7.0. The solid-state fermentation tannase at pH values of 5.0 and 6.0, supports 85-100% activity Aspergillus niger