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Literature summary for 3.1.1.20 extracted from

  • Ramos, E.L.; Mata-Gomez, M.A.; Rodriguez-Duran, L.V.; Belmares, R.E.; Rodriguez-Herrera, R.; Aguilar, C.N.
    Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam (2011), Appl. Biochem. Biotechnol., 165, 1141-1151.
    View publication on PubMed
Search for more literature for 3.1.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.82
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
2.06
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
4.06
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
4.35
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
4.47
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
4.94
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
5.06
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
5.11
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
5.17
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
12.05
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Aspergillus niger
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Aspergillus niger GH1
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger GH1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation and G25 Sephadex gel filtration Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
 Aspergillus niger gallate + methanol
-
 ?
methyl gallate + H2O
-
 Aspergillus niger GH1 gallate + methanol
-
 ?
tannic acid + H2O
-
 Aspergillus niger gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Aspergillus niger GH1 gallate + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 at a methyl gallate concentration of 4.4 mM Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 65
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.02
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
52.48
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
60.99
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
78.01
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
580.1
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
601.4
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
1109
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
1364
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
1564
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
2611
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 30°C Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 8
-
 Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.45
-
 methyl gallate at 20°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
10.26
-
 methyl gallate at 25°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
12.06
-
 methyl gallate at 35°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
15.08
-
 methyl gallate at 30°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
216.7
-
 methyl gallate at 65°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
273.5
-
 methyl gallate at 50°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
282
-
 methyl gallate at 40°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
313.7
-
 methyl gallate at 55°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
330
-
 methyl gallate at 45°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger
350.2
-
 methyl gallate at 60°C, in 50 mM citrate buffer at pH 5.0 Aspergillus niger