Application | Comment | Organism |
---|---|---|
food industry | the use of Lactobacillus plantarum tannase is an adequate alternative to the fungal tannases currently used in the food industry. Use of tannase may provide an efficient means for obtaining molecules with valuable activities from the degradation of complex tannins present in food and agricultural wastes | Lactiplantibacillus plantarum |
Cloned (Comment) | Organism |
---|---|
1.4 kb purified PCR product of tanLp1 gene cloned into pURI3 vector and transformed into Escherichia coli DH5alpha cells. Recombinant plasmids isolated and overexpressed in Escherichia coli JM109 (DE3) | Lactiplantibacillus plantarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 1 mM inhibits activity by 85% | Lactiplantibacillus plantarum | |
ethyl gallate | 1 mM inhibits activity by 48% | Lactiplantibacillus plantarum | |
Hg2+ | 1 mM inhibits activity by 78% | Lactiplantibacillus plantarum | |
Mg2+ | 1 mM inhibits activity by 12% | Lactiplantibacillus plantarum | |
additional information | 1 mM DMSO has no significant effect | Lactiplantibacillus plantarum | |
Triton X 100 | 1 mM inhibits activity by 20% | Lactiplantibacillus plantarum | |
Tween | 1 mM inhibits activity by 20% | Lactiplantibacillus plantarum | |
Urea | 1 mM inhibits activity by 52% | Lactiplantibacillus plantarum | |
Zn2+ | 1 mM inhibits activity by 18% | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM increases activity by 81% | Lactiplantibacillus plantarum | |
K+ | 1 mM increases activity by 26% | Lactiplantibacillus plantarum | |
additional information | 1 mM EDTA has no significant effect | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on affinity column | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epigallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | gallic acid + epigallocatechin | - |
? | |
ethyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + ethanol | - |
? | |
ethyl gallate + H2O | - |
Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | gallic acid + ethanol | - |
? | |
gallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + gallocatechin | - |
? | |
gallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | gallic acid + gallocatechin | - |
? | |
lauryl gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + lauric acid | - |
? | |
methyl gallate + H2O | recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate | Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
methyl gallate + H2O | recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate | Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | gallate + methanol | - |
? | |
additional information | tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized | Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 | ? | - |
? | |
propyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallic acid + propanol | - |
? | |
protocatechuic acid ethyl ester + H2O | - |
Lactiplantibacillus plantarum | protocatechuate + ethanol | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallic acid + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tannase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant tannase | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
purified recombinant tannase | Lactiplantibacillus plantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Lactiplantibacillus plantarum |