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Literature summary for 3.1.1.20 extracted from
- Effects of temperature, pH and additives on the activity of tannase produced by a co-culture of Rhizopus oryzae and Aspergillus foetidus (2006), World J. Microbiol. Biotechnol., 22, 207-212.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Aspergillus foetidus |  |
| Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Rhizopus arrhizus | |
| Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Aspergillus foetidus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Aspergillus foetidus | |
| 1,10-phenanthroline | - |
Rhizopus arrhizus | |
| 2-mercaptoethanol | - |
Aspergillus foetidus | |
| 2-mercaptoethanol | - |
Rhizopus arrhizus | |
| Ba2+ | - |
Aspergillus foetidus | |
| Ba2+ | - |
Rhizopus arrhizus | |
| Ca2+ | - |
Aspergillus foetidus | |
| Ca2+ | - |
Rhizopus arrhizus | |
| Cu2+ | - |
Aspergillus foetidus | |
| Cu2+ | - |
Rhizopus arrhizus | |
| EDTA | - |
Aspergillus foetidus | |
| EDTA | - |
Rhizopus arrhizus | |
| Fe3+ | - |
Aspergillus foetidus | |
| Fe3+ | - |
Rhizopus arrhizus | |
| Hg2+ | - |
Aspergillus foetidus | |
| Hg2+ | - |
Rhizopus arrhizus | |
| Mn2+ | - |
Aspergillus foetidus | |
| Mn2+ | - |
Rhizopus arrhizus | |
| Triton X-100 | - |
Aspergillus foetidus | |
| Triton X-100 | - |
Rhizopus arrhizus | |
| Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Aspergillus foetidus | |
| Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Rhizopus arrhizus | |
| Zn2+ | - |
Aspergillus foetidus | |
| Zn2+ | - |
Rhizopus arrhizus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
Tannic acid | pH 5.0, 40°C | Rhizopus arrhizus | |
| 0.21 | - |
Tannic acid | pH 5.0, 40°C | Aspergillus foetidus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Rhizopus arrhizus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Rhizopus arrhizus | - |
gallate + ? | - |
? | |
| tannic acid + H2O | Aspergillus foetidus | - |
gallate + ? | - |
? | |
| tannic acid + H2O | Rhizopus arrhizus RO IIT RB-13 | - |
gallate + ? | - |
? | |
| tannic acid + H2O | Aspergillus foetidus GMRB 013 MTCC 3557 | - |
gallate + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus foetidus | - |
- |
- |
| Aspergillus foetidus GMRB 013 MTCC 3557 | - |
- |
- |
| Rhizopus arrhizus | - |
- |
- |
| Rhizopus arrhizus RO IIT RB-13 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by acetone precipitation and anion exchange chromatography | Rhizopus arrhizus |
| native enzyme by acetone precipitation and anion exchange chromatography | Aspergillus foetidus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | co-culture with Aspergillus foetidus, solid-state fermentation on tannin rich substrates | Rhizopus arrhizus | - |
| additional information | co-culture with Rhizopus oryzae, solid-state fermentation on tannin rich substrates | Aspergillus foetidus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | - |
Rhizopus arrhizus | gallate + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus foetidus | gallate + ? | - |
? | |
| tannic acid + H2O | - |
Rhizopus arrhizus RO IIT RB-13 | gallate + ? | - |
? | |
| tannic acid + H2O | - |
Aspergillus foetidus GMRB 013 MTCC 3557 | gallate + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
enzyme from co-culture with Aspergillus foetidus | Rhizopus arrhizus |
| 40 | - |
enzyme from co-culture with Rhizopus oryzae | Aspergillus foetidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 55 | activity range | Rhizopus arrhizus |
| 5 | 55 | activity range | Aspergillus foetidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivation of purified enzyme after 255 min | Rhizopus arrhizus |
| 40 | - |
inactivation of purified enzyme after 255 min | Aspergillus foetidus |
| 60 | - |
inactivation of purified enzyme after 105 min | Rhizopus arrhizus |
| 60 | - |
inactivation of purified enzyme after 105 min | Aspergillus foetidus |
| 65 | - |
inactivation of purified enzyme after 75 min | Rhizopus arrhizus |
| 65 | - |
inactivation of purified enzyme after 75 min | Aspergillus foetidus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
enzyme from co-culture with Aspergillus foetidus, the tannase needs an acidic environment to be active | Rhizopus arrhizus |
| 5 | - |
enzyme from co-culture with Rhizopus oryzae, the tannase needs an acidic environment to be active | Aspergillus foetidus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | activity range | Rhizopus arrhizus |
| 3 | 6 | activity range | Aspergillus foetidus |
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