Literature summary for 3.1.1.2 extracted from

Navarro-Gonzalez, I.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
Molecular characterization of a novel arylesterase from the wine-associated acetic acid bacterium Gluconobacter oxidans 621H (2012), J. Agric. Food Chem., 60, 10789-10795.

Search for more literature for 3.1.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Gluconobacter oxydans

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyldicarbonate	0.5 mM, complete loss of activity	Gluconobacter oxydans
eserine	0.5 mM, 42% residual activity	Gluconobacter oxydans
paraoxon	0.5 mM, complete loss of activity	Gluconobacter oxydans
Phenylglyoxal	0.5 mM, 77% residual activity	Gluconobacter oxydans
pyridoxal 5'-phosphate	0.5 mM, 62% residual activity	Gluconobacter oxydans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0052	-	3-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
0.0079	-	2-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
0.0088	-	3-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
0.01	-	phenyl acetate	pH 7.0, 25°	Gluconobacter oxydans
0.0128	-	2-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
0.0174	-	4-nitrophenyl acetate	pH 7.0, 25°	Gluconobacter oxydans
0.174	-	4-nitrophenyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
7.2	-	S-methyl thiobutanoate	pH 8.0, 25°	Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	2 * 35000, SDS-PAGE	Gluconobacter oxydans
76800	-	gel filtration	Gluconobacter oxydans

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	10% v/v, 93% residual activity	Gluconobacter oxydans
acetonitrile	10% v/v, 95% residual activity	Gluconobacter oxydans
dimethyl sulfoxide	10% v/v, 90% residual activity, 40% v/v, 45% residual activity	Gluconobacter oxydans
Ethanol	10% v/v, 92% residual activity	Gluconobacter oxydans
Methanol	10% v/v, 90% residual activity, 40% v/v, 57% residual activity	Gluconobacter oxydans
propanol	10% v/v, 90% residual activity	Gluconobacter oxydans

Organism

Organism	UniProt	Comment	Textmining
Gluconobacter oxydans	Q5FSJ2	-	-
Gluconobacter oxydans 621H	Q5FSJ2	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
214	-	pH 7.0, 25°C	Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-naphthyl acetate + H2O	-	Gluconobacter oxydans	2-naphthol + acetate	-	?
2-naphthyl acetate + H2O	-	Gluconobacter oxydans 621H	2-naphthol + acetate	-	?
2-naphthyl butanoate + H2O	-	Gluconobacter oxydans	2-naphthol + butanoate	-	?
3-naphthyl acetate + H2O	-	Gluconobacter oxydans	3-naphthol + acetate	-	?
3-naphthyl butanoate + H2O	-	Gluconobacter oxydans	3-naphthol + butanoate	-	?
4-nitrophenyl acetate + H2O	-	Gluconobacter oxydans	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Gluconobacter oxydans 621H	4-nitrophenol + acetate	-	?
4-nitrophenyl butanoate + H2O	-	Gluconobacter oxydans	4-nitrophenol + butanoate	-	?
4-nitrophenyl butanoate + H2O	-	Gluconobacter oxydans 621H	4-nitrophenol + butanoate	-	?
additional information	substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site	Gluconobacter oxydans	?	-	?
additional information	substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site	Gluconobacter oxydans 621H	?	-	?
phenyl acetate + H2O	-	Gluconobacter oxydans	phenol + acetate	-	?
phenyl acetate + H2O	-	Gluconobacter oxydans 621H	phenol + acetate	-	?
S-methyl thiobutanoate + H2O	-	Gluconobacter oxydans	methanol + thiobutanoate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE	Gluconobacter oxydans

Synonyms

Synonyms	Comment	Organism
Est0881	-	Gluconobacter oxydans
gox0881	-	Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7	-	2-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
16.4	-	3-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
20	-	S-methyl thiobutanoate	pH 8.0, 25°	Gluconobacter oxydans
55.2	-	2-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
97.2	-	4-nitrophenyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
99	-	3-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
185	-	4-nitrophenyl acetate	pH 7.0, 25°	Gluconobacter oxydans
231	-	phenyl acetate	pH 7.0, 25°	Gluconobacter oxydans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Gluconobacter oxydans

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.8	-	S-methyl thiobutanoate	pH 8.0, 25°	Gluconobacter oxydans
540	-	2-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
560	-	4-nitrophenyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
1900	-	3-naphthyl butanoate	pH 7.0, 25°	Gluconobacter oxydans
6900	-	2-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
10600	-	4-nitrophenyl acetate	pH 7.0, 25°	Gluconobacter oxydans
19100	-	3-naphthyl acetate	pH 7.0, 25°	Gluconobacter oxydans
23100	-	phenyl acetate	pH 7.0, 25°	Gluconobacter oxydans

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Release 2023.1 (January 2023)