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Literature summary for 3.1.1.2 extracted from

  • Hwang, H.; Kim, S.; Yoon, S.; Ryu, Y.; Lee, S.Y.; Kim, T.D.
    Characterization of a novel oligomeric SGNH-arylesterase from Sinorhizobium meliloti 1021 (2010), Int. J. Biol. Macromol., 46, 145-152.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PCR product inserted into pQE30 vector and expressed in Escherichia coli XL1-Blue Sinorhizobium meliloti 1021

Protein Variants

Protein Variants Comment Organism
S10A completely loses activity Sinorhizobium meliloti 1021

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Sinorhizobium meliloti 1021
ethanol with 20% ethanol and 5-10% sodium dodecyl sulfate, whereas no significant changes in presence of 10% ethanol. Mg2+ and Ca2+ have no effect Sinorhizobium meliloti 1021
Urea leads to complete unfolding at 2.0 M Sinorhizobium meliloti 1021

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
3 * 22900, sequence analysis Sinorhizobium meliloti 1021
69550
-
dynamic light scattering Sinorhizobium meliloti 1021

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti 1021
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by sonication and on Ni-NTA affinity resin Sinorhizobium meliloti 1021

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
recombinant Sm23, with 4-nitrophenyl acetate as substrate Sinorhizobium meliloti 1021

Storage Stability

Storage Stability Organism
-20°C Sinorhizobium meliloti 1021

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O shows maximum activity (100%) against p-nitrophenyl acetate Sinorhizobium meliloti 1021 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Sinorhizobium meliloti 1021 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O low activity Sinorhizobium meliloti 1021 4-nitrophenol + decanoate
-
?
alpha-naphthyl acetate + H2O
-
Sinorhizobium meliloti 1021 alpha-naphthol + acetate
-
?
beta-naphthyl acetate + H2O
-
Sinorhizobium meliloti 1021 beta-naphthol + acetate
-
?
additional information can not hydrolyze organophosphates Sinorhizobium meliloti 1021 ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 22900, sequence analysis Sinorhizobium meliloti 1021

Synonyms

Synonyms Comment Organism
SGNH-arylesterase
-
Sinorhizobium meliloti 1021
Sm23
-
Sinorhizobium meliloti 1021

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
is resistant to thermal denaturation up to 50°C. Sm23 irreversibly unfolds after heating over 60°C Sinorhizobium meliloti 1021

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sinorhizobium meliloti 1021

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
at pH 4-6 it is highly unstable and disordered, less than 40% of its maximal activity at pH 7.0 Sinorhizobium meliloti 1021

pI Value

Organism Comment pI Value Maximum pI Value
Sinorhizobium meliloti 1021
-
-
4.89

General Information

General Information Comment Organism
physiological function contains a catalytic triad (Ser10, Asp187, and His190) and oxyanion holes (Ser10-Gly50-Asn90). Spherical or globular aggregates are formed with 1-butyl-3-methylimidazolium tetrafluoroborate, while amorphous aggregates are formed with 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide Sinorhizobium meliloti 1021