Cloned (Comment) | Organism |
---|---|
application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens | |
0.18 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
0.211 | - |
2-naphthyl acetate | pH 8.0 | Oryctolagus cuniculus | |
0.26 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
0.5 | - |
2-naphthyl acetate | pH 8.0 | Mus musculus | |
0.65 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15166 | - |
- |
Mus musculus | Q62087 | - |
- |
Oryctolagus cuniculus | Q9BGN0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Homo sapiens | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Oryctolagus cuniculus | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Mus musculus | 2-naphthol + acetate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
2-naphthyl acetate | pH 8.0 | Oryctolagus cuniculus | |
1.9 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1A7 | Homo sapiens | |
2 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G1B11 | Homo sapiens | |
2.12 | - |
2-naphthyl acetate | pH 8.0 | Mus musculus | |
18.8 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G2C2 | Homo sapiens | |
26.25 | - |
2-naphthyl acetate | pH 8.0, recombinant genetic variant G3G3 | Homo sapiens |