Cloned (Comment) | Organism |
---|---|
the recombinant enzyme is expressed in Trichoplusia ni High Five insect cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, recombinant enzyme | Homo sapiens |
40000 | - |
2 * 40000, SDS-PAGE | Homo sapiens |
42000 | - |
3 * 42000, recombinant enzyme, SDS-PAGE | Homo sapiens |
91900 | - |
serum PON1, nondenaturing PAGE | Homo sapiens |
94900 | - |
recombinant enzyme, nondenaturing PAGE | Homo sapiens |
95600 | - |
recombinant PON1, nondenaturing PAGE | Homo sapiens |
127500 | - |
recombinant enzyme, nondenaturing PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27169 | - |
- |
Homo sapiens | Q15165 | - |
- |
Homo sapiens | Q15166 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood serum | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.086 | - |
pH 8.0, 25°C, substrate: phenyl acetate, purified recombinant enzyme | Homo sapiens |
0.7 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl acetate, purified recombinant enzyme | Homo sapiens |
0.96 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme | Homo sapiens |
1.3 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme | Homo sapiens |
1.4 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme | Homo sapiens |
4.1 | - |
pH 8.0, 25°C, substrate: phenyl acetate, purified recombinant enzyme | Homo sapiens |
11.4 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme | Homo sapiens |
13.6 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme | Homo sapiens |
15 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl acetate, purified recombinant enzyme | Homo sapiens |
20.7 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme | Homo sapiens |
39 | - |
pH 8.0, 25°C, substrate: 4-nitrophenyl acetate, purified recombinant enzyme | Homo sapiens |
1120 | - |
pH 8.0, 25°C, substrate: phenyl acetate, purified recombinant enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 6 months, less than 15% loss of activity | Homo sapiens |
4°C, purified recombinant PON1 preparations typically lose about 20% of the arylesterase activity within the first month after purification, compared with less than 10% loss for the enzyme purified from serum. There is no further significant loss of the PON1 activity afterward, suggesting the existence of two pools in the purified PON1, a labile one and a stable one | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | activity is 2.6fold higher than that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | activity is about 40% of the activity with PON3 (Q15166 (UniProt)) | Homo sapiens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | activity is about 5% of that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | activity is similar to that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl propionate + H2O | activity is 1.5fold higher than that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | activity is 8.7fold higher than that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | activity is about 10% of the activity with PON3 (Q15166 (UniProt)) | Homo sapiens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | activity is about 65% of the activity with PON3 (Q15166 (UniProt)) | Homo sapiens | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | activity is about 7% of that with PON1 (P27169 (UniProt)) | Homo sapiens | 4-nitrophenol + propionate | - |
? | |
phenyl acetate + H2O | activity is less than 1% of that with PON1 (P27169 (UniProt)) | Homo sapiens | phenol + acetate | - |
? | |
phenyl acetate + H2O | activity is more than 100fold higher than with PON2 (Q15165 (UniProt)) and PON3 (Q15166 (UniProt)) | Homo sapiens | phenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Homo sapiens |
dimer | 2 * 39000, recombinant enzyme | Homo sapiens |
trimer | 3 * 42000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |