Literature summary for 3.1.1.17 extracted from

Chen, C.N.; Chin, K.H.; Wang, A.H.; Chou, S.H.
The first crystal structure of gluconolactonase important in the glucose secondary metabolic pathways (2008), J. Mol. Biol., 384, 604-614.

Search for more literature for 3.1.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, using 0.1 M imidazole (pH 8.0), 0.25 M Ca(OAc)2, and 15% PEG 3350 as a precipitant	Xanthomonas campestris

Protein Variants

Protein Variants	Comment	Organism
E48A	mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme	Xanthomonas campestris
N134A	mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme	Xanthomonas campestris
N191	mutant shows only marginal activities of approximately 10% toward D-glucono-1,5-lactone compared to that of the wild type enzyme	Xanthomonas campestris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0162	-	D-glucono-1,5-lactone	in 1 mM potassium phosphate buffer (pH 7.0) with 0.075 mM CaCl2	Xanthomonas campestris

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	contains two calcium ions	Xanthomonas campestris

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris	B0RN69	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Xanthomonas campestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucono-1,5-lactone + H2O	XC5397 exhibits lactonase activity only toward D-glucono-1,5-lactone	Xanthomonas campestris	D-gluconate	-	?
additional information	XC5397 does not hydrolyze D-gulono-1,4-lactone, L-gulono-1,4-lactone, and D-ribono-1,4-lactone	Xanthomonas campestris	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Xanthomonas campestris

Synonyms

Synonyms	Comment	Organism
GNL	-	Xanthomonas campestris
SMP-30	-	Xanthomonas campestris
XC5397	-	Xanthomonas campestris

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Release 2023.1 (January 2023)