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Literature summary for 3.1.1.17 extracted from

  • Ishikawa, T.; Nishikawa, H.; Gao, Y.; Sawa, Y.; Shibata, H.; Yabuta, Y.; Maruta, T.; Shigeoka, S.
    The pathway via D-galacturonate/L-galactonate is significant for ascorbate biosynthesis in Euglena gracilis: identification and functional characterization of aldonolactonase (2008), J. Biol. Chem., 283, 31133-31141.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.17

Activating Compound

Activating Compound Comment Organism Structure
hydrogen peroxide stimulates activity at 0.1-0.5 mM Euglena gracilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
EDTA strong inhibition Euglena gracilis
additional information not inhibited by L-ascorbic acid and dithiothreitol Euglena gracilis
N-ethylmaleimide
-
 Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.55
-
 L-galactonic acid recombinant enzyme Euglena gracilis
1.67
-
 L-galactono-1,4-lactone recombinant enzyme Euglena gracilis
2.08
-
 D-gulono-1,5-lactone recombinant enzyme Euglena gracilis
3.05
-
 L-gulono-1,4-lactone recombinant enzyme Euglena gracilis
4.55
-
 L-Gulonic acid recombinant enzyme Euglena gracilis
9.4
-
 D-glucono-1,5-lactone wild type enzyme Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mn2+, Co2+, Mg2+, and Ca2+ have a significant low effect on the activity to 19, 9, 8, and 7%, respectively Euglena gracilis
Zn2+ the enzyme requires a divalent metal ion for maximum activity which is obtained by the addition of 0.075 mM Zn2+ Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 about 34000 Da, recombinant enzyme, SDS-PAGE Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis A9CPS8
-
-
Euglena gracilis Z A9CPS8
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
 wild type enzyme, using L-galactono-1,4-lactone as substrate Euglena gracilis
2.7
-
 wild type enzyme, using L-gulono-1,4-lactone as substrate Euglena gracilis
5.4
-
 wild type enzyme, using L-glucono-1,5-lactone as substrate Euglena gracilis
9.4
-
 wild type enzyme, using D-galactono-1,4-lactone as substrate Euglena gracilis
12
-
 wild type enzyme, using D-gulono-1,4-lactone as substrate Euglena gracilis
20.1
-
 recombinant enzyme, using D-gulono-1,4-lactone as substrate Euglena gracilis
24.5
-
 recombinant enzyme, using L-galactono-1,4-lactone as substrate Euglena gracilis
27.3
-
 recombinant enzyme, using D-galactono-1,4-lactone as substrate Euglena gracilis
39
-
 recombinant enzyme, using D-glucono-1,5-lactone as substrate Euglena gracilis
40.9
-
 recombinant enzyme, using L-gulono-1,4-lactone as substrate Euglena gracilis
217
-
 wild type enzyme, using D-glucono-1,5-lactone as substrate Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactonic acid + H2O
-
 Euglena gracilis ?
-
 ?
D-galactonic acid + H2O
-
 Euglena gracilis Z ?
-
 ?
D-galactono-1,4-lactone + H2O wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis ?
-
 r
D-galactono-1,4-lactone + H2O wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z ?
-
 r
D-glucono-1,5-lactone + H2O wild type and recombinant enzyme show 100% specific activity Euglena gracilis D-gluconate
-
 r
D-glucono-1,5-lactone + H2O wild type and recombinant enzyme show 100% specific activity Euglena gracilis Z D-gluconate
-
 r
D-gulono-1,4-lactone + H2O wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis D-galactonate
-
 r
D-gulono-1,4-lactone + H2O wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z D-galactonate
-
 r
D-gulono-1,5-lactone + H2O
-
 Euglena gracilis ?
-
 ?
L-galactonic acid + H2O
-
 Euglena gracilis ?
-
 ?
L-galactono-1,4-lactone + H2O wild type enzyme shows 0.37% specific activity, recombinant enzyme shows 62.8% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis ?
-
 r
L-glucono-1,5-lactone + H2O wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis L-gluconate
-
 r
L-glucono-1,5-lactone + H2O wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z L-gluconate
-
 r
L-gulonic acid + H2O
-
 Euglena gracilis ?
-
 ?
L-gulono-1,4-lactone + H2O wild type enzyme shows 1.24% specific activity, recombinant enzyme shows 105% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis L-gulonate
-
 r

Synonyms

Synonyms Comment Organism
ALase
-
 Euglena gracilis
aldonolactonase
-
 Euglena gracilis
SMP30
-
 Euglena gracilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
160
-
 D-gulono-1,5-lactone recombinant enzyme Euglena gracilis
192
-
 D-glucono-1,5-lactone wild type enzyme Euglena gracilis
450
-
 L-galactono-1,4-lactone recombinant enzyme Euglena gracilis
615
-
 L-galactonic acid recombinant enzyme Euglena gracilis
1040
-
 L-gulono-1,4-lactone recombinant enzyme Euglena gracilis
1503
-
 L-Gulonic acid recombinant enzyme Euglena gracilis