Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.17 extracted from

  • Shinagawa, E.; Ano, Y.; Yakushi, T.; Adachi, O.; Matsushita, K.
    Solubilization, purification, and properties of membrane-bound D-glucono-delta-lactone hydrolase from Gluconobacter oxydans (2009), Biosci. Biotechnol. Biochem., 73, 241-244.
    View publication on PubMed
Search for more literature for 3.1.1.17

Inhibitors

Inhibitors Comment Organism Structure
EDTA highly sensitive to EDTA, the lost activity can be restored to the original level by the addition of divalent cations of the order Ca2+ = Mg2+ > Zn2+ > Fe2+ = Co2+, Mn2+, Ni2+, Cu2+ > Mo2+ Gluconobacter oxydans
EGTA highly sensisitve to EGTA, the lost activity can be restored to the original level by the addition of divalent cations of the order Ca2+ = Mg2+ > Zn2+ > Fe2+ = Co2+, Mn2+, Ni2+, Cu2+ > Mo2+ Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
 D-glucono-1,5-lactone estimated value Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gluconobacter oxydans 16020
-
soluble solubilization with 1 M CaCl2 Gluconobacter oxydans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the purified enzyme is strictly dependent on Ca2+ and undergoes rapid denaturing precipitation on Ca2+ depletion even in the presence of detergent Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, gel filtration and SDS-PAGE Gluconobacter oxydans
120000
-
 gel filtration Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans IFO 3244
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethyleneglycol 6000 precipitation and Sephadex G-75 gel filtration Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 crude extract Gluconobacter oxydans
2.5
-
 after 31.2fold purification Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucono-1,5-lactone + H2O 100% relative activity Gluconobacter oxydans D-gluconate
-
 ?
D-glucono-1,5-lactone + H2O 100% relative activity Gluconobacter oxydans IFO 3244 D-gluconate
-
 ?
D-xylono-1,5-lactone + H2O 90% relative activity compared to D-glucono-1,5-lactone Gluconobacter oxydans D-xylonate
-
 ?
D-xylono-1,5-lactone + H2O 90% relative activity compared to D-glucono-1,5-lactone Gluconobacter oxydans IFO 3244 D-xylonate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, gel filtration and SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
membrane-bound D-glucono-delta-lactone hydrolase
-
 Gluconobacter oxydans
MGL
-
 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Gluconobacter oxydans