Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.14 extracted from

  • Kermasha, S.; Khalyfa, A.; Marsot, P.; Alli, I.; Fournier, R.
    Biomass production, purification and characterization of chlorophyllase from alga (Phaeodactylum tricornutum) (1992), Biotechnol. Appl. Biochem., 15, 142-159.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
Phaeodactylum tricornutum
iodoacetamide
-
Phaeodactylum tricornutum
NEM
-
Phaeodactylum tricornutum

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid membrane
-
Phaeodactylum tricornutum 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum

Organism

Organism UniProt Comment Textmining
Phaeodactylum tricornutum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.79
-
enzyme isolated at the exponential growth phase Phaeodactylum tricornutum
134.4
-
enzyme isolated at the stationary growth phase Phaeodactylum tricornutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Phaeodactylum tricornutum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.8 pH 7.5: about 35% of maximal activity, pH 8.8: about 65% of maximal activity Phaeodactylum tricornutum