Literature summary for 3.1.1.14 extracted from

Chen, S.; Wang, S.; Huang, M.; Lin, K.; Hua, S.; Lu, H.; Lai, Y.; Yang, C.
Physiological and molecular analyses of chlorophyllase in sweet potatoes with different-colored leaves (2018), South Afr. J. Bot., 114, 272-279 .

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation	Ipomoea batatas
DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase2 in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation implying that a frame shift mutation of the Chlase gene has evolved in CYY8467	Ipomoea batatas

Cloned (Comment)

Organism

DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation

Ipomoea batatas

DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, and phylogenetic analysis and tree, the isozymes from cultivars TN57, CN1927, and CYY8467 share 95% identity, and an eight-nucleotide sequence of Chlase2 in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation implying that a frame shift mutation of the Chlase gene has evolved in CYY8467

Ipomoea batatas

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chlorophyll a + H2O	Ipomoea batatas	-	phytol + chlorophyllide a	-	?
chlorophyll b + H2O	Ipomoea batatas	-	phytol + chlorophyllide b	-	?

Organism

Organism	UniProt	Comment	Textmining
Ipomoea batatas	A0A2U8KGL8	cv. TN57; cultivar TN57 with green leaves	-
Ipomoea batatas	A0A2U8KGM2	cv. CN1927; cultivar CN1927 with yellow leaves	-
Ipomoea batatas	A0A2U8KGN4	cv. CYY8467; cultivar CYY8467 with purple leaves	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	expression of the Chlase gene is detected in all leaves, differential mRNA expressions of the enzyme in various leaves from the different cultivars, enzyme mRNA is most strongly active and regulated in cv. TN57	Ipomoea batatas	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
chlorophyll a + H2O	-	Ipomoea batatas	phytol + chlorophyllide a	-	?
chlorophyll b + H2O	-	Ipomoea batatas	phytol + chlorophyllide b	-	?
additional information	substrate specificities of isozymes from different cultivars, overview. Chlorophyllase (Chl) activity in CN1927 exhibits a higher substrate preference toward chlorophyll (Chl) b than Chl a	Ipomoea batatas	?	-	?
additional information	substrate specificities of isozymes from different cultivars, overview. Chlorophyllase (Chl) activity in cultivar CYY8467 exhibits a higher substrate preference toward chlorophyll (Chl) a than Chl b	Ipomoea batatas	?	-	?
additional information	substrate specificities of isozymes from different cultivars, overview. Chlorophyllase (Chl) activity in cultivar TN57 exhibits a slightly higher substrate preference toward chlorophyll (Chl) a than Chl b	Ipomoea batatas	?	-	?

Synonyms

Synonyms	Comment	Organism
Chlase	-	Ipomoea batatas
Chlase 2	-	Ipomoea batatas
chlorophyllase 2	-	Ipomoea batatas
CYY8467 Chlase2	-	Ipomoea batatas
IbChlase	-	Ipomoea batatas
truncated chlorophyllase 2	UniProt	Ipomoea batatas

General Information

General Information	Comment	Organism
evolution	IbChlase in TN57, CN1927, and CYY8467 shares 95% identity, and an eight-nucleotide sequence of Chlase in CYY8467 is missing due to a frame-shift mutation of the Chlase gene leading to early termination of gene translation. Sequences from cutlivars TN57 and CN1927 are clustered together in a clade as Chlase2. Phylogenetic analysis and tree	Ipomoea batatas
malfunction	the incomplete Chlase2 protein of cultivar CYY8467 lacks for the core domain, suggesting that the incomplete CYY8467 Chlase2 does not function in Chl degradation	Ipomoea batatas
physiological function	chlorophyllase (Chlase) is a hydrophobic enzyme that catalyzes the hydrolysis of chlorophyll (Chl) to chlorophyllide (Chlide) and phytol. Chlase characteristics of sweet potatoes (Ipomoea batatas) with various leaf colors indifferent varieties: yellow (CN1927), green (TN57), and purple (CYY8467). Large variations in Chl and anthocyanin (Ant) and carotenoid (Car) exist in all plants, and cv. CYY8467 contains significantly higher Chl, Ant, and Car levels compared to cvs. TN57 and CN1927. Various plants exhibit different Chlase activities, and Chl a degradation by Chlase of CYY8467 is higher than those of TN57 and CN1927	Ipomoea batatas