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Literature summary for 3.1.1.14 extracted from

  • Chou, Y.; Ko, C.; Chen, L.; Yen, C.; Shaw, J.
    Purification and immobilization of the recombinant Brassica oleracea chlorophyllase 1 (BoCLH1) on DIAION®CR11 as potential biocatalyst for the production of chlorophyllide and phytol (2015), Molecules, 20, 3744-3757 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene BOLC5T30519H, functional recombinant expression of C-terminally His-tagged enzyme BoCHL1 in Escherichia coli strain BL21(DE3) Brassica oleracea

Protein Variants

Protein Variants Comment Organism
additional information usage of a polystyrene divinylbenzene-based metal chelator to purify and immobilize recombinant poly(His)-tagged recombinant BoCLH1. Cu2+ exhibits the highest capacity and purification efficiency, therefore DIAION®CR11Cu(II) is the preferred resin for enzyme immobilization. Enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme. Enzyme immobilized on resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme Brassica oleracea

General Stability

General Stability Organism
enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea Q8GTM4
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme BoCHL1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll + H2O substrate ethanol-dissolved Chl a from Anacystis nidulans algae Brassica oleracea phytol + chlorophyllide
-
 ?

Subunits

Subunits Comment Organism
? x * 38630, SDS-PAGE Brassica oleracea

Synonyms

Synonyms Comment Organism
BoCLH1
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 Brassica oleracea
BOLC5T30519H
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 Brassica oleracea
Chlase
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 Brassica oleracea
chlorophyllase 1
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 Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Brassica oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 90 purified enzyme, 100 mM sodium phosphate, pH 7.0 with 0.24% Triton X-100, 30 min Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Brassica oleracea