Cloned (Comment) | Organism |
---|---|
gene BOLC5T30519H, functional recombinant expression of C-terminally His-tagged enzyme BoCHL1 in Escherichia coli strain BL21(DE3) | Brassica oleracea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | usage of a polystyrene divinylbenzene-based metal chelator to purify and immobilize recombinant poly(His)-tagged recombinant BoCLH1. Cu2+ exhibits the highest capacity and purification efficiency, therefore DIAION®CR11Cu(II) is the preferred resin for enzyme immobilization. Enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme. Enzyme immobilized on resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme | Brassica oleracea |
General Stability | Organism |
---|---|
enzyme immobilized on His-Trap resin is about 2fold stabilized at 60°C and during 17 cycles of use compared to free enzyme | Brassica oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | Q8GTM4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme BoCHL1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | substrate ethanol-dissolved Chl a from Anacystis nidulans algae | Brassica oleracea | phytol + chlorophyllide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38630, SDS-PAGE | Brassica oleracea |
Synonyms | Comment | Organism |
---|---|---|
BoCLH1 | - |
Brassica oleracea |
BOLC5T30519H | - |
Brassica oleracea |
Chlase | - |
Brassica oleracea |
chlorophyllase 1 | - |
Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Brassica oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | purified enzyme, 100 mM sodium phosphate, pH 7.0 with 0.24% Triton X-100, 30 min | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Brassica oleracea |