Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.14 extracted from

  • Chou, Y.L.; Ko, C.Y.; Yen, C.C.; Chen, L.F.; Shaw, J.F.
    A novel recombinant chlorophyllase1 from Chlamydomonas reinhardtii for the production of chlorophyllide derivatives (2015), J. Agric. Food Chem., 63, 9496-9503 .
    View publication on PubMed
Search for more literature for 3.1.1.14

Application

Application Comment Organism
synthesis recombinant enzyme CrCLH1 can be used as a biocatalyst to produce chlorophyllide derivatives Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
gene CLH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant C-terminally GFP-tagged enzyme expression driven by a CAMV35S promoter is determined in chloroplasts of Arabidopsis thaliana mesophyll protoplasts (isolated from 3-4-week-old well-expanded leaves yet to flower), recombinant overexpression of soluble N-terminally T7-tagged enzyme in Escherichia coli strain BL21(DE3) Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0566
-
 chlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
0.0999
-
 chlorophyll b recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
0.4603
-
 bacteriochlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll + H2O Chlamydomonas reinhardtii
-
 phytol + chlorophyllide
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii A8J2S9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant T7-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyll a + H2O
-
 Chlamydomonas reinhardtii phytol + bacteriochlorophyllide a
-
 ?
chlorophyll + H2O
-
 Chlamydomonas reinhardtii phytol + chlorophyllide
-
 ?
chlorophyll a + H2O preferred substrate Chlamydomonas reinhardtii phytol + chlorophyllide a
-
 ?
chlorophyll b + H2O
-
 Chlamydomonas reinhardtii phytol + chlorophyllide b
-
 ?
additional information recombinant CrCLH1 has a higher catalytic efficiency for chlorophyll a than for chlorophyll b and bacteriochlorophyll a. Recombinant CrCLH1 catalyzes the conversion of chlorophyll a to pheophorbide a at pH 5.0 Chlamydomonas reinhardtii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36500, recombinant T7-tagged enzyme, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
Chlase
-
 Chlamydomonas reinhardtii
chlorophyllase 1
-
 Chlamydomonas reinhardtii
chlorophyllase1
-
 Chlamydomonas reinhardtii
CLH1
-
 Chlamydomonas reinhardtii
CrCLH1
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Chlamydomonas reinhardtii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75 recombinant enzyme, activity range, profile overview. 90% of maximal activity at 20°C and 45°C, 70% at 50°C, 25% at 70°C, inactivation at 80°C Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 purified recombinant enzyme, completely stable up to, 35% activity remaining at 70°C, inactivation at 80°C Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
212.2
-
 bacteriochlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
232.8
-
 chlorophyll b recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
559.55
-
 chlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 over 50% of maximal activity within this range Chlamydomonas reinhardtii
5 7 over 90% of maximal activity within this range Chlamydomonas reinhardtii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 purified recombinant enzyme, stability profile, overview, over 50% activity remaining at pH 4.5-9.0, over 90% at pH 7.0, completely stable at pH 5.0-6.0 Chlamydomonas reinhardtii

General Information

General Information Comment Organism
evolution the enzyme shares th highly conserved lipase motif GXSXG Chlamydomonas reinhardtii
physiological function recombinant chlorophyllase 1, CrCLH1, can perform chlorophyll dephytylation and produce chlorophyllide and phytol Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
461
-
 bacteriochlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
2330
-
 chlorophyll b recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii
9886
-
 chlorophyll a recombinant enzyme, pH 7.0, 60°C Chlamydomonas reinhardtii