Application | Comment | Organism |
---|---|---|
synthesis | the enzyme CyanoCLH can be used as a biocatalyst for the production of a bacteriochlorin a (BCA) precursor by degrading bacteriochlorophyll a (BChl a). Bacteriopheophorbide a (BPheid a) is used as a precursor for bacteriochlorin a (BCA), which can be used for photodynamic therapy in both in vitro and in vivo biochemical applications | Crocosphaera subtropica ATCC 51142 |
Cloned (Comment) | Organism |
---|---|
gene cce_5094, sequence comparisons, recombinant expression of the N-terminally T7-tagged enzyme (T7 tag sequence is MASMTGGQQMG)in Escherichia coli strain BL21(DE3) | Crocosphaera subtropica ATCC 51142 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, Michaelis-Menten kinetics | Crocosphaera subtropica ATCC 51142 | |
0.0495 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
0.157 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
0.2795 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacteriochlorophyll a + H2O | Crocosphaera subtropica ATCC 51142 | preferred substrate | phytol + bacteriochlorophyllide a | - |
? | |
chlorophyll + H2O | Crocosphaera subtropica ATCC 51142 | - |
phytol + chlorophyllide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crocosphaera subtropica ATCC 51142 | B1X2S9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.88 | - |
purified recombinant enzyme, pH 7.0, 60°C, substrate chlorophyll b | Crocosphaera subtropica ATCC 51142 |
11.15 | - |
purified recombinant enzyme, pH 7.0, 60°C, substrate chlorophyll a | Crocosphaera subtropica ATCC 51142 |
75.6 | - |
purified recombinant enzyme, pH 7.0, 60°C, substrate bacteriochlorophyll a | Crocosphaera subtropica ATCC 51142 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bacteriochlorophyll a + H2O | preferred substrate | Crocosphaera subtropica ATCC 51142 | phytol + bacteriochlorophyllide a | - |
? | |
chlorophyll + H2O | - |
Crocosphaera subtropica ATCC 51142 | phytol + chlorophyllide | - |
? | |
chlorophyll a + H2O | - |
Crocosphaera subtropica ATCC 51142 | phytol + chlorophyllide a | - |
? | |
chlorophyll b + H2O | - |
Crocosphaera subtropica ATCC 51142 | phytol + chlorophyllide b | - |
? | |
additional information | enzyme substrate specificity, overview. Recombinant CyanoCLH prefers hydrolyzing bacteriochlorophyll a to produce bacteriochlorophyllide a (BChlide a), which can be converted to bacteriopheophorbide a by removing magnesium ion | Crocosphaera subtropica ATCC 51142 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54700, recombinant T7-tagged enzyme, SDS-PAGE | Crocosphaera subtropica ATCC 51142 |
Synonyms | Comment | Organism |
---|---|---|
cce_5094 | - |
Crocosphaera subtropica ATCC 51142 |
Chlase | - |
Crocosphaera subtropica ATCC 51142 |
CyanoCLH | - |
Crocosphaera subtropica ATCC 51142 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | 80% of maximal activity, recombinant enzyme | Crocosphaera subtropica ATCC 51142 |
60 | - |
assay at | Crocosphaera subtropica ATCC 51142 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
90% activity remains at 70°C, the relative activity of the recombinant CyanoCLH decreases sharply at 80-90°C | Crocosphaera subtropica ATCC 51142 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
251.08 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
310.36 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
726.33 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Crocosphaera subtropica ATCC 51142 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 85% of maximal activity, recombinant enzyme | Crocosphaera subtropica ATCC 51142 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | purified recombinant enzyme, over 80% activity remaining | Crocosphaera subtropica ATCC 51142 |
General Information | Comment | Organism |
---|---|---|
evolution | the deduced amino acid sequence of recombinant CyanoCLH comprises a unique lipase-motif GHSLG, which differs from the GHSRG sequence of other plants and lacks a histidine of the typical and conserved catalytic triad Ser-Asp-His | Crocosphaera subtropica ATCC 51142 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
chlorophyll b | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
1599 | - |
chlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 | |
14688 | - |
bacteriochlorophyll a | recombinant enzyme, pH 7.0, 60°C | Crocosphaera subtropica ATCC 51142 |