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Literature summary for 3.1.1.14 extracted from

  • Yang, C.H.; Yen, C.C.; Jheng, J.J.; Wang, C.Y.; Chen, S.S.; Huang, P.Y.; Huang, K.S.; Shaw, J.F.
    Immobilization of Brassica oleracea chlorophyllase 1 (BoCLH1) and Candida rugosa lipase (CRL) in magnetic alginate beads: an enzymatic evaluation in the corresponding proteins (2014), Molecules, 19, 11800-11815.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll a + H2O Brassica oleracea
-
phytol + chlorophyllide a
-
?

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.68
-
free enzyme, at pH 6.0 and 40°C Brassica oleracea
8.52
-
enzyme immobilized in alginate, at pH 6.0 and 40°C Brassica oleracea
10.9
-
enzyme immobilized in magnetic iron oxide nanoparticles-alginate, at pH 6.0 and 40°C Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll a + H2O
-
Brassica oleracea phytol + chlorophyllide a
-
?

Synonyms

Synonyms Comment Organism
CHL1
-
Brassica oleracea
chlorophyllase 1
-
Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Brassica oleracea