Cloned (Comment) | Organism |
---|---|
expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Triticum aestivum | |
Cu2+ | - |
Triticum aestivum | |
diisopropyl fluorophosphate | - |
Triticum aestivum | |
Fe2+ | - |
Triticum aestivum | |
Fe3+ | - |
Triticum aestivum | |
Hg2+ | - |
Triticum aestivum | |
iodoacetamide | - |
Triticum aestivum | |
Mg2+ | - |
Triticum aestivum | |
additional information | inhibitory effects on recombinant enzymes purified by microtiter plate method and by affinity chromatography, respectively, overview, no inhibition by iodoacetic acid, iodoacetamide, 2-mercaptoethanol, and DTT | Triticum aestivum | |
N-ethylmaleimide | - |
Triticum aestivum | |
phenylmethanesulfonyl fluoride | - |
Triticum aestivum | |
Zn2+ | - |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state kinetics, the recombinant immobilized fusion protein displays kinetic parameters similar to those of recombinant enzyme purified by affnity chromatography, overview | Triticum aestivum | |
0.05 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography | Triticum aestivum | |
0.069 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll + H2O | Triticum aestivum | chlorophyllase catalyzes the initial hydrolysis of the phytol moiety from the pigment in the degradation of chlorophyll, overview | phytol + chlorophyllide | - |
? | |
additional information | Triticum aestivum | since chlorophyll degradation is a defining feature of plant senescence, compounds inhibiting chlorophyllase activity may delay senescence, thereby improving shelf life and appearance of plant products | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, or by using a microtiter plate purification method with immobilization of the enzyme, overview | Triticum aestivum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a continuous assay system, replacing chlorophyll with p-nitrophenyl-ester substrates eliminates the extraction step and allows for continuous measurement of chlorophyllase activity in the multiwell plate format, overview | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Triticum aestivum | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
Triticum aestivum | 4-nitrophenol + decanoate | - |
? | |
chlorophyll + H2O | - |
Triticum aestivum | phytol + chlorophyllide | - |
? | |
chlorophyll + H2O | chlorophyllase catalyzes the initial hydrolysis of the phytol moiety from the pigment in the degradation of chlorophyll, overview | Triticum aestivum | phytol + chlorophyllide | - |
? | |
additional information | since chlorophyll degradation is a defining feature of plant senescence, compounds inhibiting chlorophyllase activity may delay senescence, thereby improving shelf life and appearance of plant products | Triticum aestivum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Triticum aestivum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.05 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by affinity chromatography | Triticum aestivum | |
9.43 | - |
4-nitrophenyl decanoate | pH 8.0, 25°C, recombinant enzyme purified by microtiter plate method | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, substrate chlorophyll | Triticum aestivum |
8 | - |
assay at, substrate 4-nitrophenyl esters | Triticum aestivum |