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Literature summary for 3.1.1.13 extracted from
- Amperometric determination of serum total cholesterol with nanoparticles of cholesterol esterase and cholesterol oxidase (2016), Anal. Biochem., 500, 6-11 .
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | glutaraldehyde cross-linked and functionalized cholesterol esterase and cholesterol oxidase nanoparticles (ChENPs) can be used as biosensors. The biosensor exhibits optimal response at pH 5.5 and 40°C within 5 s when polarized at +0.25 V versus Ag/AgCl. The working/linear range of the biosensor is 10-700 mg/dl for cholesterol. The sensor shows high sensitivity and measures total cholesterol as low as 0.1 mg/dl. The biosensor is evaluated and employed for total cholesterol determination in sera of apparently healthy and diseased persons. Measurement of total cholesterol level in serum is very important due to its presumed relationship to atherosclerosis and cardiovascular diseases, nephritis, myxedema, obstructive jaundice, diabetes mellitus, and cerebral thrombosis | Sus scrofa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme by desolvation with ethanol and subsequent cross-linking with glutaraldehyde, preparation of glutaraldehyde cross-linked and functionalized cholesterol esterase nanoparticles (ChENPs), co-immobilization of cholesterol oxidase, method optimization and evaluation, overview | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Sus scrofa | - |
| pancreas | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl acetate + H2O | - |
Sus scrofa | cholesterol + acetate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChE | - |
Sus scrofa |
| cholesterol esterase | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Sus scrofa |
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Release 2023.1 (January 2023)
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