Literature summary for 3.1.1.13 extracted from

  • Xiang, H.; Masuo, S.; Hoshino, T.; Takaya, N.
    Novel family of cholesterol esterases produced by actinomycetes bacteria (2007), Biochim. Biophys. Acta, 1774, 112-120.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.3%, 1.55fold activation Streptomyces sp.
cholic acid 0.3%, 1.5fold activation Streptomyces avermitilis
Tween 80 0.3%, 3fold activation Streptomyces avermitilis
Triton X-100 0.3%, 5fold activation Streptomyces griseus
Triton X-100 0.3%, 5fold activation Streptomyces avermitilis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 77% residual activity Streptomyces avermitilis
Ag+ 1 mM, 81% residual activity Streptomyces avermitilis
Ag+ 1 mM, 80% residual activity Streptomyces griseus
Ag+ 1 mM, 75% residual activity Streptomyces sp.
cholic acid 0.3%, 50% residual activity Streptomyces griseus
cholic acid 0.3%, 85% residual activity Streptomyces sp.
deoxycholic acid 0.3%, 40% residual activity Streptomyces avermitilis
deoxycholic acid 0.3%, 25% residual activity Streptomyces griseus
deoxycholic acid 0.3%, 33% residual activity Streptomyces sp.
dithiothreitol 1 mM, complete loss of activity Streptomyces avermitilis
dithiothreitol 1 mM, complete loss of activity Streptomyces griseus
dithiothreitol 1 mM, complete loss of activity Streptomyces sp.
Hg2+ 1 mM, complete loss of activity Streptomyces avermitilis
Hg2+ 1 mM, 40% residual activity Streptomyces griseus
Hg2+ 1 mM, 50% residual activity Streptomyces sp.
KCl 1 mM, 90% residual activity Streptomyces sp.
p-chloromercuribenzoic acid 1 mM, 75% residual activity Streptomyces avermitilis
p-chloromercuribenzoic acid 1 mM, 87% residual activity Streptomyces griseus
p-chloromercuribenzoic acid 1 mM, 78% residual activity Streptomyces sp.
Tween 20 0.3%, 25% residual activity Streptomyces avermitilis
Tween 20 0.3%, 50% residual activity Streptomyces griseus
Tween 20 0.3%, 45% residual activity Streptomyces sp.
Tween 80 0.3%, 90% residual activity Streptomyces griseus
Tween 80 0.3%, 30% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces griseus
0.21
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces avermitilis
0.49
-
cholesteryl myristate pH 7.0, 37°C Streptomyces avermitilis
1
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces avermitilis
0.8
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces avermitilis
0.35
-
cholesteryl myristate pH 7.0, 37°C Streptomyces griseus
0.77
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces griseus
0.63
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces griseus
0.85
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces griseus
0.53
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces avermitilis
1.67
-
cholesteryl stearate pH 7.0, 37°C Streptomyces griseus
2.86
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, 120% of initial activity Streptomyces griseus
Cu2+ 1 mM, 120% of initial activity Streptomyces avermitilis
Cu2+ 1 mM, 120% of initial activity Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Streptomyces avermitilis
153000
-
gel filtration Streptomyces griseus
19700
-
x * 19700, SDS-PAGE Streptomyces avermitilis
25700
-
x * 25700, SDS-PAGE Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-
Streptomyces sp. Q2AAT6
-
-
Streptomyces avermitilis Q82JC7 strain JCM5070
-
Streptomyces avermitilis JCM 5070 Q82JC7 strain JCM5070
-
Streptomyces sp. X9 Q2AAT6
-
-
Streptomyces griseus IFO 13350
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces griseus
-
Streptomyces avermitilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.5
-
substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces griseus
33
-
substrate cholesteryl linoleate, pH 7.0, 37°C Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl palmitate + H2O
-
Streptomyces griseus cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Streptomyces avermitilis cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Streptomyces sp. cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Streptomyces sp. X9 cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces griseus 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces avermitilis 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces sp. 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces avermitilis JCM 5070 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces sp. X9 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Streptomyces griseus IFO 13350 4-nitrophenol + palmitate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces griseus 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces avermitilis 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces sp. 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces avermitilis JCM 5070 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces sp. X9 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Streptomyces griseus IFO 13350 4-nitrophenol + laurate
-
?
cholesteryl acetate + H2O
-
Streptomyces griseus cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Streptomyces avermitilis cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Streptomyces sp. cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Streptomyces sp. X9 cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + acetate
-
?
cholesteryl linoleate + H2O
-
Streptomyces griseus cholesterol + linoleate
-
?
cholesteryl linoleate + H2O
-
Streptomyces avermitilis cholesterol + linoleate
-
?
cholesteryl linoleate + H2O
-
Streptomyces sp. cholesterol + linoleate
-
?
cholesteryl linoleate + H2O
-
Streptomyces avermitilis JCM 5070 cholesterol + linoleate
-
?
cholesteryl linoleate + H2O
-
Streptomyces sp. X9 cholesterol + linoleate
-
?
cholesteryl linoleate + H2O
-
Streptomyces griseus IFO 13350 cholesterol + linoleate
-
?
cholesteryl stearate + H2O
-
Streptomyces griseus cholesterol + stearate
-
?
cholesteryl stearate + H2O
-
Streptomyces avermitilis cholesterol + stearate
-
?
cholesteryl stearate + H2O
-
Streptomyces sp. cholesterol + stearate
-
?
cholesteryl myristate + H2O
-
Streptomyces griseus cholesterol + myristate
-
?
cholesteryl myristate + H2O
-
Streptomyces avermitilis cholesterol + myristate
-
?
cholesteryl myristate + H2O
-
Streptomyces sp. cholesterol + myristate
-
?
4-nitrophenyl myristate + H2O
-
Streptomyces griseus 4-nitrophenol + myristate
-
?
4-nitrophenyl myristate + H2O
-
Streptomyces avermitilis 4-nitrophenol + myristate
-
?
4-nitrophenyl myristate + H2O
-
Streptomyces sp. 4-nitrophenol + myristate
-
?

Subunits

Subunits Comment Organism
oligomer x * 19700, SDS-PAGE Streptomyces avermitilis
oligomer x * 25700, SDS-PAGE Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.73
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces griseus
23.3
-
cholesteryl stearate pH 7.0, 37°C Streptomyces griseus
24.7
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces avermitilis
12.4
-
cholesteryl myristate pH 7.0, 37°C Streptomyces avermitilis
13.3
-
cholesteryl myristate pH 7.0, 37°C Streptomyces griseus
2.52
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces avermitilis
25.6
-
cholesteryl linoleate pH 7.0, 37°C Streptomyces griseus
2.56
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces avermitilis
3.43
-
4-nitrophenyl laurate pH 7.0, 37°C Streptomyces griseus
15.7
-
cholesteryl palmitate pH 7.0, 37°C Streptomyces avermitilis
1.44
-
4-nitrophenyl palmitate pH 7.0, 37°C Streptomyces avermitilis
3.86
-
4-nitrophenyl myristate pH 7.0, 37°C Streptomyces griseus