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Literature summary for 3.1.1.13 extracted from

  • Sugihara, A.; Shimada, Y.; Nomura, A.; Terai, T.; Imayasu, M.; Nagai, Y.; Nagao, T.; Watanabe, Y.; Tominaga, Y.
    Purification and characterization of a novel cholesterol esterase from Pseudomonas aeruginosa, with its application to cleaning lipid-stained contact lenses (2002), Biosci. Biotechnol. Biochem., 66, 2347-2355.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cholate up to 100 mM, enhancement of the activity, bile salt is absolutely required for activity Pseudomonas aeruginosa
taurocholate up to 100 mM, enhancement of the activity, bile salt is absolutely required for activity Pseudomonas aeruginosa

Application

Application Comment Organism
additional information in presence of taurocholate the enzyme is an effective cleaner for contact lenses stained with lipids consisting of cholesteryl oleate, tripalmitin, and stearyl stearate Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by iodoacetic acid, DTNB, EDTA and tetranitromethane Pseudomonas aeruginosa
PMSF
-
Pseudomonas aeruginosa
Triton X-100
-
Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent metal ions do not influence the enzyme activity Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
gel filtration Pseudomonas aeruginosa
58000
-
x * 58000, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
116fold Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O low activity, 6% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + acetate
-
?
cholesteryl butyrate + H2O low activity, 8% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + butyrate
-
?
cholesteryl caprate + H2O 40% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + caprate
-
?
cholesteryl caproate + H2O 22% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + caproate
-
?
cholesteryl caprylate + H2O 62% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + caprylate
-
?
cholesteryl laurate + H2O 41% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + laurate
-
?
cholesteryl linoleate + H2O best substrate Pseudomonas aeruginosa cholesterol + linoleate
-
?
cholesteryl myristate + H2O 58% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + myristate
-
?
cholesteryl oleate + H2O 92% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + oleate
-
?
cholesteryl palmitate + H2O 75% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + palmitate
-
?
cholesteryl stearate + H2O 80% of the activity with cholesteryl linoleate Pseudomonas aeruginosa cholesterol + stearate
-
?
triolein + H2O positional specificity for sn-2-position, lower affinity for the sn-1- and sn-3-position Pseudomonas aeruginosa diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
30 min, pH 7.0, stable Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing
-
3.2