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Literature summary for 3.1.1.13 extracted from
- Isolation of two forms of carboxylester lipase (cholesterol esterase) from porcine pancreas (1987), Biochim. Biophys. Acta, 918, 106-114.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
native and denaturating PAGE, low-molecular weight form | Sus scrofa |
| 83000 | - |
native and denaturating PAGE, high-molecular weight form | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Sus scrofa | absoprtion of free cholesterol | cholesterol + fatty acid | - |
r |  |
| fat-soluble vitamin esters + H2O | Sus scrofa | - |
vitamin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by chromatography techniques | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.2 | - |
for cholesteryl oleate | Sus scrofa |
| 19.5 | - |
for p-nitrophenyl acetate | Sus scrofa |
| 53.3 | - |
for high-molecular weight form | Sus scrofa |
| 60.5 | - |
for low-molecular weight form | Sus scrofa |
| 333 | - |
for tributyrin | Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 6 months | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | absoprtion of free cholesterol | Sus scrofa | cholesterol + fatty acid | - |
r | |
| cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
r | |
| fat-soluble vitamin esters + H2O | - |
Sus scrofa | vitamin + ? | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Sus scrofa | p-nitrophenol + acetic acid | - |
? | |
| tributyrin + H2O | - |
Sus scrofa | dibutyrylglycerol + butanoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 83000, native and denaturating PAGE | Sus scrofa |
| monomer | 1 * 74000, native and denaturating PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxylester lipase | - |
Sus scrofa |
| carboxylesterase | - |
Sus scrofa |
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