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Literature summary for 3.1.1.13 extracted from
- Characterization of multiple forms of cholesteryl ester hydrolase in the rat testis (1984), J. Biol. Chem., 259, 7433-7438.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| follicle-stimulating hormone | 26fold activation after 14 days | Rattus sp. | |
| luteinizing hormone | stimualtes only temperature-stable enzyme | Rattus sp. | |
| sodium cholate | - |
Sus scrofa |  |
| sodium cholate | maximal activation at 0.03-0.05% v/v | Rattus sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cholesteryl esters | inhibition at 0.2 mM with cholesteryl-stearate, -oleate, -eicosenoate, -arachidonate, -erucate and -nervonate | Rattus sp. | |
| sodium cholate | 50% inhibition at 0.05-0.1% v/v | Rattus sp. | |
| sodium deoxycholate | complete inhibition | Rattus sp. | |
| Sodium dodecyl sulfate | complete inactivation | Rattus sp. | |
| Triton X-100 | complete inactivation | Rattus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel permeation, temperature-stable enzyme | Rattus sp. |
| 72000 | - |
gel filtration, temperature-labile enzyme | Rattus sp. |
| 420000 | - |
gel filtration, temperature-labile enzyme | Rattus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Sus scrofa | - |
cholesterol + fatty acid | - |
? | |
| cholesteryl esters + H2O | Rattus sp. | involvement in inhibition of spermatogenesis | cholesterol + fatty acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus sp. | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by HPLC separation of temperature-labile and temperature-stable enzymes | Rattus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Rattus sp. | - |
| Leydig cell | temperature-stabile enzyme enriched | Rattus sp. | - |
| liver | - |
Rattus sp. | - |
| pancreas | - |
Sus scrofa | - |
| pancreas | - |
Rattus sp. | - |
| Sertoli cell | temperature-labile enzyme enriched | Rattus sp. | - |
| testis | temperature-labile enzyme exclusively localized | Rattus sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl arachidonate + H2O | - |
Sus scrofa | cholesterol + arachidonic acid | - |
? | |
| cholesteryl arachidonate + H2O | - |
Rattus sp. | cholesterol + arachidonic acid | - |
? | |
| cholesteryl eicosenoate + H2O | - |
Sus scrofa | cholesterol + eicosenoic acid | - |
? | |
| cholesteryl eicosenoate + H2O | - |
Rattus sp. | cholesterol + eicosenoic acid | - |
? | |
| cholesteryl erucate + H2O | - |
Sus scrofa | cholesterol + erucic acid | 13-docosenoic acid | ? | |
| cholesteryl erucate + H2O | - |
Rattus sp. | cholesterol + erucic acid | 13-docosenoic acid | ? | |
| cholesteryl esters + H2O | - |
Sus scrofa | cholesterol + fatty acid | - |
? | |
| cholesteryl esters + H2O | involvement in inhibition of spermatogenesis | Rattus sp. | cholesterol + fatty acid | - |
? | |
| cholesteryl nervonate + H2O | - |
Sus scrofa | cholesterol + nervonic acid | - |
? | |
| cholesteryl nervonate + H2O | especially for temperature-labile cholesteryl esterase | Rattus sp. | cholesterol + nervonic acid | - |
? | |
| cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
? | |
| cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
? | |
| cholesteryl stearate + H2O | - |
Sus scrofa | cholesterol + stearic acid | - |
? | |
| cholesteryl stearate + H2O | - |
Rattus sp. | cholesterol + stearic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Sus scrofa |
| dimer | 1 * 28000 SDS-PAGE, gel filtration (HPLC gel permeation) | Rattus sp. |
| multimer | - |
Sus scrofa |
| multimer | multimeric monomers of 72000 each, SDS-PAGE, gel filtration (HPLC gel permeation) temperature-labile enzyme | Rattus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
temperature-labile enzyme | Rattus sp. |
| 37 | - |
temperature-stable enzyme | Rattus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
inactivation by evaluation from 32°C to 37°C | Rattus sp. |
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