General Stability | Organism |
---|---|
50% loss of activity after storage at 4°C, 2 weeks | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5-dithiobis(2-nitrobenzoic acid) | 80% inhibition at 10 mM | Rattus sp. | |
Ca2+ | complete inactivation at 10 mM | Rattus sp. | |
Mg2+ | 44% inhibition at 5 mM | Rattus sp. | |
N-ethylmaleimide | 72% inhibition at 3 mM | Rattus sp. | |
NaF | 65% inhibition at 5 mM | Rattus sp. | |
p-chloromercuribenzoate | 76% inhibition at 1 mM | Rattus sp. | |
Protamine sulfate | 18% inhibition at 500 mg/ml | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Rattus sp. | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration, SDS-PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | Rattus sp. | hydrolytic activity toward liposomal and microcrystallic substrates | cholesterol + fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
90% purity by digitonin solubilization of lysosome-enriched pellets, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.018 | - |
for p-nitrophenylacetate | Rattus sp. |
0.31 | - |
for p-nitrophenylmyristate | Rattus sp. |
Storage Stability | Organism |
---|---|
4°C, several days | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | hydrolytic activity toward liposomal and microcrystallic substrates | Rattus sp. | cholesterol + fatty acid | - |
? | |
cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
? | |
p-nitrophenyl acetate + H2O | - |
Rattus sp. | p-nitrophenol + acetic acid | - |
? | |
p-nitrophenyllactate + H2O | - |
Rattus sp. | p-nitrophenol + lactic acid | - |
? | |
p-nitrophenylmyristate + H2O | - |
Rattus sp. | p-nitrophenol + myristic acid | - |
? | |
triolein + H2O | - |
Rattus sp. | dioleoylglycerol + oleic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE, gel filtration, also active as aggregates | Rattus sp. |