Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium taurocholic acid | absolutely required, glycocholate can fully be substituted | Rattus sp. | |
Trihydroxy bile salts | lead to conversion of enzyme tetramers into monomers | Rattus sp. |
General Stability | Organism |
---|---|
freezing and storage at -80°C deactivate, addition of bovine serum albumin increases activity | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | 0.1% v/v, hydrolysis not detectable | Rattus sp. | |
N-ethylmaleimide | complete loss of activity | Rattus sp. | |
Triton X-100 | 0.1% v/v, 12% substrate hydrolyzed in the presence of bile salts | Rattus sp. | |
Tween 40 | 0.1% v/v, hydrolysis not detectable | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
cholesteryl oleate | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Rattus sp. | |
Mg2+ | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | Rattus sp. | - |
cholesterol + fatty acid | - |
ir |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | reduces hydrolysis rate significantly | Rattus sp. |
Glycerol | inactivates | Rattus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3step chromatography techniques, preparative electrophoresis to homogeneity | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.34 | - |
- |
Rattus sp. |
Storage Stability | Organism |
---|---|
0°C, 1 week | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | - |
Rattus sp. | cholesterol + fatty acid | - |
ir | |
cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 65000, native PAGE, SDS-PAGE, activity also in monomers | Rattus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Rattus sp. |