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Literature summary for 3.1.1.13 extracted from

  • Winkler, K.E.; Harrison, E.H.; Marsh, J.B.; Glick, J.M.
    Characterization of a bile salt-dependent cholesteryl ester hydrolase activity secreted from HepG2 cells (1992), Biochim. Biophys. Acta, 1126, 151-158.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glycocholate slightly activation at 40 mM Homo sapiens
sodium cholate
-
Rattus sp.
sodium cholate activates optimal at 40 mM Homo sapiens
sodium taurocholic acid
-
Rattus sp.
sodium taurocholic acid slightly activation at 40 mM Homo sapiens

General Stability

General Stability Organism
highly unstable at 4°C Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
brefeldin A inhibition to 80%-90% of secreted enzyme into the medium of HepG2 cells Homo sapiens
monensin inhibition to 50%-60% of secreted enzyme into the medium of HepG2 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
cholesteryl oleate in presence of 40 mM sodium cholate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Homo sapiens mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats cholesterol + fatty acid
-
?
cholesteryl esters + H2O Rattus sp. mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats cholesterol + fatty acid
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-
liver hepatoma cells HepG2 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
-
Rattus sp.
0.002
-
activity after 6 h in cell homogenate Homo sapiens
0.002 0.009 activities of secreted enzyme from 0 h up to 102 h Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 0.02 mM bovine serum albumin, 20 h Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl esters + H2O mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats Homo sapiens cholesterol + fatty acid
-
?
cholesteryl esters + H2O mediating cholesterol absorption through the gastrointestinal tract, digestion and absorption of dietary fats Rattus sp. cholesterol + fatty acid
-
?
cholesteryl oleate + H2O
-
Homo sapiens cholesterol + oleic acid
-
?
cholesteryl oleate + H2O
-
Rattus sp. cholesterol + oleic acid
-
?

Synonyms

Synonyms Comment Organism
bile salt-dependent cholesteryl ester hydrolase
-
Homo sapiens
bile salt-dependent cholesteryl ester hydrolase
-
Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Homo sapiens