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Literature summary for 3.1.1.117 extracted from

  • Conacher, C.G.; Garcia-Aparicio, M.P.; Coetzee, G.; van Zyl, W.H.; Goergens, J.F.
    Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris (2019), BMC Res. Notes, 12, 596 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.117

Application

Application Comment Organism
biofuel production glucuronoyl esterase (GE) has potential for production of biofuels and biomaterials in lignocellulose biorefineries. GE cleaves alkali-labile bonds from the lignincarbohydrate complexes at acidic pH. Removal of glucuronic acid branches from the hemicellulose significantly improved release of fermentable sugars for biofuels production Trichoderma reesei

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Pichia pastoris strain DSMZ 70382 (CBS704), the recombinant enzyme is secreted Trichoderma reesei

Protein Variants

Protein Variants Comment Organism
additional information use of a constitutive PGAP-Pichia pastoris (glyceraldehyde-3-phosphate dehydrogenase promoter) expression system to produce Hypocrea jecorina glucuronoyl esterase (GE) at bioreactor scale: glycerol fed-batch fermentation kinetics of the recombinant enzyme, enzymatic yields, kinetics and productivity for bioreactor cultivations of recombinant Pichia pastoris at the end of the glycerol fed-batch stage, and comparisons to previous expression attempts, method evaluation, overview Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 4-O-methyl-D-glucopyranuronate ester + H2O Trichoderma reesei
-
 4-O-methyl-D-glucuronic acid + an alcohol
-
 ?
a 4-O-methyl-D-glucopyranuronate ester + H2O Trichoderma reesei QM6a
-
 4-O-methyl-D-glucuronic acid + an alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei G0RV93 Trichoderma reesei
-
Trichoderma reesei QM6a G0RV93 Trichoderma reesei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 4-O-methyl-D-glucopyranuronate ester + H2O
-
 Trichoderma reesei 4-O-methyl-D-glucuronic acid + an alcohol
-
 ?
a 4-O-methyl-D-glucopyranuronate ester + H2O
-
 Trichoderma reesei QM6a 4-O-methyl-D-glucuronic acid + an alcohol
-
 ?

Synonyms

Synonyms Comment Organism
4-O-methyl-glucuronoyl methylesterase UniProt Trichoderma reesei
glucuronoyl esterase
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 in vivo assay at Trichoderma reesei