Any feedback?
Literature summary for 3.1.1.117 extracted from
- beta-Glucuronidase-coupled assays of glucuronoyl esterases (2016), Anal. Biochem., 510, 114-119 .
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | glucuronoyl esterases are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-D-glucuronic acid in plant cell walls. This activity renders glucuronoyl esterases attractive research targets for biotechnological applications. One of the factors impeding the progress in glucuronoyl esterases research is the lack of suitable substrates. A facile preparation is described of methyl esters of chromogenic 4-nitrophenyl and 5-bromo-4-chloro-3-indolyl beta-D-glucuronides for qualitative and quantitative glucuronoyl esterase assay coupled with beta-glucuronidase as the auxiliary enzyme. The indolyl derivative affording a blue indigo-type product is suitable for rapid and sensitive assay of glucuronoyl esterase in commercial preparations as well as for high throughput screening of microorganisms and genomic and metagenomic libraries | Trichoderma reesei |
| biotechnology | glucuronoyl esterases are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-D-glucuronic acid in plant cell walls. This activity renders glucuronoyl esterases attractive research targets for biotechnological applications. One of the factors impeding the progress in glucuronoyl esterases research is the lack of suitable substrates. A facile preparation is described of methyl esters of chromogenic 4-nitrophenyl and 5-bromo-4-chloro-3-indolyl beta-D-glucuronides for qualitative and quantitative glucuronoyl esterase assay coupled with beta-glucuronidase as the auxiliary enzyme. The indolyl derivative affording a blue indigo-type product is suitable for rapid and sensitive assay of glucuronoyl esterase in commercial preparations as well as for high throughput screening of microorganisms and genomic and metagenomic libraries | Ruminococcus flavefaciens |
| biotechnology | glucuronoyl esterases are microbial enzymes with potential to cleave the ester bonds between lignin alcohols and xylan-bound 4-O-methyl-D-glucuronic acid in plant cell walls. This activity renders glucuronoyl esterases attractive research targets for biotechnological applications. One of the factors impeding the progress in glucuronoyl esterases research is the lack of suitable substrates. A facile preparation is described of methyl esters of chromogenic 4-nitrophenyl and 5-bromo-4-chloro-3-indolyl beta-D-glucuronides for qualitative and quantitative glucuronoyl esterase assay coupled with beta-glucuronidase as the auxiliary enzyme. The indolyl derivative affording a blue indigo-type product is suitable for rapid and sensitive assay of glucuronoyl esterase in commercial preparations as well as for high throughput screening of microorganisms and genomic and metagenomic libraries | Schizophyllum commune |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminococcus flavefaciens | Q9RLB8 | - |
- |
| Schizophyllum commune | D8QLP9 | - |
- |
| Schizophyllum commune H4-8 | D8QLP9 | - |
- |
| Trichoderma reesei | G0RV93 | i.e.Trichoderma reesei | - |
| Trichoderma reesei QM6a | G0RV93 | i.e.Trichoderma reesei | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl (4-nitrophenyl beta-D-glucopyranosid)uronate + H2O | - |
Trichoderma reesei | 4-nitrophenyl beta-D-glucopyranosiduronic acid + methanol | - |
? |  |
| methyl (4-nitrophenyl beta-D-glucopyranosid)uronate + H2O | - |
Ruminococcus flavefaciens | 4-nitrophenyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (4-nitrophenyl beta-D-glucopyranosid)uronate + H2O | - |
Schizophyllum commune | 4-nitrophenyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (4-nitrophenyl beta-D-glucopyranosid)uronate + H2O | - |
Schizophyllum commune H4-8 | 4-nitrophenyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (4-nitrophenyl beta-D-glucopyranosid)uronate + H2O | - |
Trichoderma reesei QM6a | 4-nitrophenyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (5-bromo-4-chloro-3-indolyl beta-D-glucopyranosid)uronate + H2O | - |
Trichoderma reesei | 5-bromo-4-chloro-3-indolyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (5-bromo-4-chloro-3-indolyl beta-D-glucopyranosid)uronate + H2O | - |
Ruminococcus flavefaciens | 5-bromo-4-chloro-3-indolyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (5-bromo-4-chloro-3-indolyl beta-D-glucopyranosid)uronate + H2O | - |
Schizophyllum commune | 5-bromo-4-chloro-3-indolyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (5-bromo-4-chloro-3-indolyl beta-D-glucopyranosid)uronate + H2O | - |
Schizophyllum commune H4-8 | 5-bromo-4-chloro-3-indolyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
| methyl (5-bromo-4-chloro-3-indolyl beta-D-glucopyranosid)uronate + H2O | - |
Trichoderma reesei QM6a | 5-bromo-4-chloro-3-indolyl beta-D-glucopyranosiduronic acid + methanol | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
