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Literature summary for 3.1.1.117 extracted from
- Identification of genes encoding microbial glucuronoyl esterases (2007), FEBS Lett., 581, 4029-4035 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Hypocrea jecorina | Trichoderma reesei |
| gene cip2, sequence and domain comparisons, functional recombinant overexpression of enzyme Cip2 under cellulase-induction conditions in Hypocrea jecorina strain Rut C-30 using the cel7A promoter, the cel5A signal peptide and carbohydrate-binding module (CBM)-coding region, and the Escherichia coli hygromycin B phosphotransferase gene. The recombinant enzyme is secreted | Trichoderma reesei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA up to 5 mM | Trichoderma reesei | |
| PMSF | 1 mM, 10 min, 20% loss of activity. 5 mM, 10 mon, 80% loss of activity; 5-10 mM, over 80% inhibition | Trichoderma reesei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 5.5, 30°C | Trichoderma reesei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma reesei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | G0RV93 | - |
- |
| Trichoderma reesei | G0RV93 | Trichoderma reesei | - |
| Trichoderma reesei QM6a | G0RV93 | - |
- |
| Trichoderma reesei QM6a | G0RV93 | Trichoderma reesei | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | family 1 CBM sequences are found in enzyme TrCip2, N-glycosylation site N447QS might be glycosylated. N- and/or O-glycosylation is commonly found for secreted proteins by Hypocrea jecorina | Trichoderma reesei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme Cip2 from overexpressing Hypocrea jecorina strain Rut C-30 to homogeneity by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(4-methoxyphenyl)propyl methyl 4-O-methyl-alpha-D-glucopyranosiduronate + H2O | a chromogenic substrate | Trichoderma reesei | methanol + 3-(4-methoxyphenyl)propyl 4-O-methyl-alpha-D-glucopyranosiduronate | - |
? | |
| 3-(4-methoxyphenyl)propyl methyl 4-O-methyl-alpha-D-glucopyranosiduronate + H2O | a chromogenic substrate | Trichoderma reesei QM6a | methanol + 3-(4-methoxyphenyl)propyl 4-O-methyl-alpha-D-glucopyranosiduronate | - |
? | |
| 3-(4-methoxyphenyl)propyl-methyl-4-O-methyl-alpha-D-glucopyranuronate + H2O | substrate mimicking the ligninhemicellulose linkage | Trichoderma reesei | methanol + 3-(4-methoxyphenyl)propyl-4-O-methyl-alpha-D-glucopyranuronate | - |
? | |
| 3-(4-methoxyphenyl)propyl-methyl-4-O-methyl-alpha-D-glucopyranuronate + H2O | substrate mimicking the ligninhemicellulose linkage | Trichoderma reesei QM6a | methanol + 3-(4-methoxyphenyl)propyl-4-O-methyl-alpha-D-glucopyranuronate | - |
? | |
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | a chromogenic substrate | Trichoderma reesei | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | - |
Trichoderma reesei QM6a | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | a chromogenic substrate | Trichoderma reesei QM6a | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| methyl 4-O-methyl-D-glucopyranuronate + H2O | - |
Trichoderma reesei | methanol + 4-O-methyl-D-glucopyranuronate | - |
? | |
| methyl 4-O-methyl-D-glucopyranuronate + H2O | - |
Trichoderma reesei QM6a | methanol + 4-O-methyl-D-glucopyranuronate | - |
? | |
| O-methyl-alpha-D-glucopyranuronate + H2O | - |
Trichoderma reesei | methanol + alpha-D-glucuronic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46400, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Trichoderma reesei |
| monomer | 1 * 46400, calculated, 1 * 55000, SDS-PAGE | Trichoderma reesei |
| More | the mass of the Cip2 CBM and linker (unglycosylated) domains is 3.73 kDa and 3.92 kDa, respectively. Peptide mapping, pepitdes are identified by HPLC/tandem mass spectrometry after trypsin digestion of Cip2 | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-O-methyl-glucuronoyl methylesterase | UniProt | Trichoderma reesei |
| Cip2 | - |
Trichoderma reesei |
| glucuronoyl esterase | - |
Trichoderma reesei |
| TrCip2 | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | - |
Trichoderma reesei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | - |
Trichoderma reesei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 5.5, 30°C | Trichoderma reesei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Trichoderma reesei |
| 5.5 | - |
substrate 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | Trichoderma reesei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichoderma reesei | isoelectric focusing | - |
7.09 |
| Trichoderma reesei | isoelectric focusing | - |
7.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Trichoderma reesei | up-regulated under conditions of induction of cellulolytic and hemicellulolytic enzymes | up |
| Trichoderma reesei | the cip2 gene of Hypocrea jecorina is upregulated under conditions of induction of cellulolytic and hemicellulolytic enzymes | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure homology modelling | Trichoderma reesei |
| physiological function | Cip2 protein is a bimodular protein in which the catalytic module is connected to a carbohydrate binding module of family 1 | Trichoderma reesei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 5.5, 30°C | Trichoderma reesei | |
| 9.7 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 5.5, 30°C | Trichoderma reesei | |
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