Literature summary for 3.1.1.117 extracted from

Katsimpouras, C.; Benarouche, A.; Navarro, D.; Karpusas, M.; Dimarogona, M.; Berrin, J.G.; Christakopoulos, P.; Topakas, E.
Enzymatic synthesis of model substrates recognized by glucuronoyl esterases from Podospora anserina and Myceliophthora thermophila (2014), Appl. Microbiol. Biotechnol., 98, 5507-5516 .

Search for more literature for 3.1.1.117

Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermothelomyces thermophilus
expression in Pichia pastoris	Podospora anserina
gene ge2, functional recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain X-33 under transcriptional control of alcohol oxidase (AOX1) promoter	Thermothelomyces thermophilus
gene page1, functional recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain X-33 under transcriptional control of alcohol oxidase (AOX1) promoter	Podospora anserina

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular docking of substrates	Thermothelomyces thermophilus
molecular docking of substrates	Podospora anserina

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	Michaelis-Menten kinetics	Podospora anserina
additional information	-	additional information	StGE2 hydrolysis results towards ester VI, a substrate analogue with a hydroxyl substituent on benzene ring, are unable to fit in Michaelis-Menten function, as plateau is not achieved up to substrate concentrations of 8.0 mM, the Km value is estimated erroneously high, indicating low specificity of StGE2 for substrate VI	Thermothelomyces thermophilus
0.94	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
0.94	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
1.34	-	3-(4-hydroxyphenyl)-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
1.34	-	3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
2.66	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
2.66	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
3.63	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
3.63	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus
7.24	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
7.24	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Podospora anserina	-	-

Organism

Organism	UniProt	Comment	Textmining
Podospora anserina	B2ABS0	-	-
Podospora anserina	B2ABS0	Pleurage anserina	-
Podospora anserina ATCC MYA-4624	B2ABS0	Pleurage anserina	-
Podospora anserina DSM 980	B2ABS0	-	-
Podospora anserina DSM 980	B2ABS0	Pleurage anserina	-
Podospora anserina FGSC 10383	B2ABS0	Pleurage anserina	-
Podospora anserina S	B2ABS0	Pleurage anserina	-
Thermothelomyces thermophilus	G2QJR6	-	-
Thermothelomyces thermophilus	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus ATCC 42464	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus BCRC 31852	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus DSM 1799	G2QJR6	-	-
Thermothelomyces thermophilus DSM 1799	G2QJR6	Sporotrichum thermophile	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme GE1 possesses a N-terminal family 1 carbohydrate-binding module (CBM) and a catalytic domain, linked by a hinge region. No potential N-glycosylation sites are identified along the whole protein sequence, but 22 O-glycosylation sites are predicted located in the linker region upstream of the catalytic domain of PaGE1	Podospora anserina

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Pichia pastoris by nickel affinity chromatography to homogeneity	Thermothelomyces thermophilus
recombinant His6-tagged enzyme from Pichia pastoris by nickel affinity chromatography to homogeneity	Podospora anserina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3-(4-hydroxyphenyl)-1-propyl D-glucopyranosyluronate + H2O	-	Podospora anserina	?	-	?
3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina	3-(4-hydroxyphenyl)-propan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina ATCC MYA-4624	3-(4-hydroxyphenyl)-propan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina DSM 980	3-(4-hydroxyphenyl)-propan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina FGSC 10383	3-(4-hydroxyphenyl)-propan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina S	3-(4-hydroxyphenyl)-propan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenyl-1-propyl D-glucopyranosyluronate + H2O	-	Thermothelomyces thermophilus	?	-	?
3-phenyl-1-propyl D-glucopyranosyluronate + H2O	-	Podospora anserina	?	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus ATCC 42464	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina ATCC MYA-4624	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina DSM 980	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina FGSC 10383	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus BCRC 31852	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina S	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus DSM 1799	3-phenylpropan-1-ol + 4-O-methyl-D-glucopyranuronate	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Thermothelomyces thermophilus	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Podospora anserina	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Thermothelomyces thermophilus ATCC 42464	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Podospora anserina ATCC MYA-4624	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Podospora anserina DSM 980	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Podospora anserina FGSC 10383	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Thermothelomyces thermophilus BCRC 31852	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Podospora anserina S	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Thermothelomyces thermophilus DSM 1799	methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus ATCC 42464	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina ATCC MYA-4624	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina DSM 980	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina FGSC 10383	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus BCRC 31852	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina S	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
cinnamyl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus DSM 1799	cinnamyl alcohol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Thermothelomyces thermophilus	methanol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina	methanol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina ATCC MYA-4624	methanol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina DSM 980	methanol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina FGSC 10383	methanol + 4-O-methyl-D-glucopyranuronate	-	?
methyl 4-O-methyl-D-glucopyranuronate + H2O	-	Podospora anserina S	methanol + 4-O-methyl-D-glucopyranuronate	-	?
additional information	no substrate: 3-(4-hydroxyphenyl)-1-propyl 4-O-methyl-D-glucopyranuronate	Thermothelomyces thermophilus	?	-	?
additional information	molecular docking of model substrates in the catalytic site of StGE2	Thermothelomyces thermophilus	?	-	-
additional information	no substrate: 3-(4-hydroxyphenyl)-1-propyl 4-O-methyl-D-glucopyranuronate	Thermothelomyces thermophilus ATCC 42464	?	-	?
additional information	molecular docking of model substrates in the catalytic site of StGE2	Thermothelomyces thermophilus ATCC 42464	?	-	-
additional information	no substrate: 3-(4-hydroxyphenyl)-1-propyl 4-O-methyl-D-glucopyranuronate	Thermothelomyces thermophilus BCRC 31852	?	-	?
additional information	molecular docking of model substrates in the catalytic site of StGE2	Thermothelomyces thermophilus BCRC 31852	?	-	-
additional information	no substrate: 3-(4-hydroxyphenyl)-1-propyl 4-O-methyl-D-glucopyranuronate	Thermothelomyces thermophilus DSM 1799	?	-	?
additional information	molecular docking of model substrates in the catalytic site of StGE2	Thermothelomyces thermophilus DSM 1799	?	-	-
trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate + H2O	-	Thermothelomyces thermophilus	?	-	?
trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate + H2O	-	Podospora anserina	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 50942, calculated, x * 63000, SDS-PAGE of recombinant protein	Podospora anserina
?	x * 63000, recombinant enzyme, SDS-PAGE, x * 51700, about, sequence calculation	Podospora anserina
More	the sequence predicts a family 1 carbohydrate-binding module (CBM1) (22-59), a linker peptide (60-105), and a catalytic CE15 module (106-481)	Podospora anserina

Synonyms

Synonyms	Comment	Organism
4-O-methyl-glucuronoyl methylesterase	-	Podospora anserina
GE1	-	Podospora anserina
Ge2	-	Thermothelomyces thermophilus
glucuronoyl esterase	-	Thermothelomyces thermophilus
glucuronoyl esterase	-	Podospora anserina
glucuronoyl esterase 1	-	Podospora anserina
glucuronoyl esterase 2	-	Thermothelomyces thermophilus
MYCTH_55568	locus name	Thermothelomyces thermophilus
PaGE1	-	Podospora anserina
podansg148	locus name	Podospora anserina
StGE2	-	Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Thermothelomyces thermophilus
55	-	-	Podospora anserina

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.19	-	3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
0.19	-	3-(4-hydroxyphenyl)-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
0.77	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
0.78	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
1.93	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus
1.93	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
2.77	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus
2.77	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
5.25	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
5.26	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Thermothelomyces thermophilus
7	-	assay at	Podospora anserina

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	high activity at	Podospora anserina

pI Value

Organism	Comment	pI Value Maximum	pI Value
Podospora anserina	sequence calculation	-	6.9
Podospora anserina	isolelectric focusing, isoform 1	-	7.6
Podospora anserina	isolelectric focusing, isoform 2	-	8.2
Podospora anserina	calculated	-	8.5

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the carbohydrate esterase family, CE15	Thermothelomyces thermophilus
evolution	the enzyme belongs to the carbohydrate esterase family, CE15. The conserved sequence G-C-S-R-N-G contains the catalytic nucleophile serine as component of the catalytic site of CE15 hydrolases	Podospora anserina
additional information	docking calculations using the crystal structure of GE2 S213A mutant from Myceliophthora thermophila in complex with methyl 4-O-methyl-beta-D-glucopyranuronate (PDB code 4G4J) as template. Enzyme GE1 possesses a N-terminal family 1 carbohydrate-binding module (CBM) and a catalytic domain, linked by a hinge region. The conserved sequence G-C-S-R-N-G contains the catalytic nucleophile serine as component of the catalytic site	Podospora anserina
additional information	molecular docking calculations using the crystal structure of GE2 S213A mutant from Myceliophthora thermophila in complex with methyl 4-O-methyl-beta-D-glucopyranuronate (PDB code 4G4J) as template	Thermothelomyces thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.14	-	3-(4-hydroxyphenyl)prop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
0.14	-	3-(4-hydroxyphenyl)-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
0.38	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus
0.38	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
0.53	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Thermothelomyces thermophilus
0.53	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Thermothelomyces thermophilus
0.82	-	3-phenylprop-1-yl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
0.82	-	3-phenyl-1-propyl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina
1.97	-	cinnamyl 4-O-methyl-D-glucopyranuronate	pH 6.0, 50°C	Podospora anserina
1.98	-	trans-3-phenyl-2-propen-1-yl D-glucopyranosyluronate	pH 7.0, 50°C, recombinant enzyme	Podospora anserina