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Literature summary for 3.1.1.11 extracted from

  • Levesque-Tremblay, G.; Mueller, K.; Mansfield, S.D.; Haughn, G.W.
    Highly methyl esterified seeds is a pectin methyl esterase involved in embryo development (2015), Plant Physiol., 167, 725-737 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
gene PME6, DNA and amino acid sequence determination and analysis, recombinant expression of YFP-tagged enzyme in the seed coat and cells of the embryos, the HMS-YFP fusions does not have enough HMS activity to compensate for the hms mutation Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a hms-1 mutant from gene PME6 by a transposon insertion. The hms-1 embryo defect phenotype is caused by a mutation in HMS, molecular complementation is performed, phenotype overview. Analysis of a second hms allele, allele gabi-kat-278G11, i.e. hms-2. The T-DNA in hms-2 is inserted at 229 from the +1 site in the 5' UTR. The hms-2 mutant has no mucilage phenotype, and HMS transcript in hms-2 is comparable with the wild-type. No other alleles are available in the coding region of HMS Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
additional information endogenous inhibitor PMEI5 does not act through HMS in the embryo cell wall Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Arabidopsis thaliana 5618
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pectin + n H2O Arabidopsis thaliana
-
 n methanol + pectate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49298 ecotypes Nos-0 and Col-0
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information analysis of seed anatomy and embryo cell sizes throughout development of wild-type and hms-1 mutant Arabidopsis thaliana
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plant embryo
-
 Arabidopsis thaliana
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seed coat
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + n H2O
-
 Arabidopsis thaliana n methanol + pectate
-
 ?

Synonyms

Synonyms Comment Organism
At1g23200 locus name Arabidopsis thaliana
HMS
-
 Arabidopsis thaliana
hms-1
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 Arabidopsis thaliana
pectin methyl esterase
-
 Arabidopsis thaliana
PME6
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a defect in mucilage extrusion is observed in a PME6 mutant and is shown to be a pleiotropic effect of the changes in embryo. hms-1 Embryo defect phenotype, the embryo cell size is decreased, the hms-1 radicals and cotyledons both have a reduced cell perimeter compared with the wild-type, overview. The PME activity is decreased and the degree of methyl esterification is increased in hms-1 7-DPA mutant seeds Arabidopsis thaliana
physiological function highly methyl esterified seeds' (gene PME6) is a pectin methyl esterase involved in embryo development, it is required for normal embryo development. Enzyme HMS causes the softening of plant tissues. Isozyme HMS plays an important role in embryo growth Arabidopsis thaliana