Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epigallocatechin | 82% residual activity at 20 mg/ml | Aspergillus flavus | |
catechinhydrate | 83% residual activity at 20 mg/ml | Aspergillus flavus | |
epigallocatechin gallate | 47% residual activity at 20 mg/ml | Aspergillus flavus | |
gallic acid | 80% residual activity at 20 mg/ml | Aspergillus flavus | |
additional information | 10 mg/ml sucrose and 10 mg/ml coumaric acid have no effect on enzyme activity | Aspergillus flavus | |
SDS | 78% residual activity at 10 mg/ml SDS | Aspergillus flavus | |
Tannic acid | 10 mg/ml tannic acid completely inhibits the enzyme | Aspergillus flavus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Aspergillus flavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Aspergillus flavus | - |
methanol + pectate | - |
? | |
pectin + H2O | Aspergillus flavus NRRL3357 | - |
methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Aspergillus flavus NRRL3357 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HisTrap column chromatography, and SOURCE 30Q column chromatography | Aspergillus flavus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.7 | - |
crude enzyme, at pH 5.0 and 30°C | Aspergillus flavus |
194 | - |
after 34fold purification, at pH 5.0 and 30°C | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus flavus | methanol + pectate | - |
? | |
pectin + H2O | - |
Aspergillus flavus NRRL3357 | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Aspergillus flavus |
Synonyms | Comment | Organism |
---|---|---|
pectin methylesterase | - |
Aspergillus flavus |
PME | - |
Aspergillus flavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus flavus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | the enzyme activity is slightly increased at temperatures above 30°C, but peaks at 55°C. At temperatures above 55°C, the activity drops rapidly, and no activity is detected at 65°C | Aspergillus flavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Aspergillus flavus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 6 | - |
Aspergillus flavus |